Document Detail

Bivariate flow karyotyping with air-cooled lasers.
MedLine Citation:
PMID:  7523043     Owner:  NLM     Status:  MEDLINE    
An experimental flow cytometer was constructed using a quartz flow cell optically coupled to a 1.22 NA lens. A pair of crossed cylindrical quartz lenses allowed multilaser excitation. Two helium-cadmium (HeCd) lasers, emitting 16 mW at 442 nm and 35 mW at 325 nm, were used to excite chromomycin A3 and Hoechst 33258 fluorescence, respectively. Bivariate flow karyotypes from normal human male chromosomes and from the Daudi cell line were obtained and were compared to those from a standard instrument using dual water-cooled lasers. The new experimental instrument exhibited comparable resolution to that from the standard instrument. In further experiments with Daudi chromosomes, the 35 mW HeCd laser was replaced with a 10 mW HeCd laser, and the system still gave good, though slightly decreased, resolution.
T Frey; D W Houck; B J Shenker; R A Hoffman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytometry     Volume:  16     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-10-28     Completed Date:  1994-10-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  169-74     Citation Subset:  IM    
Becton Dickinson Immunocytometry Systems, San Jose, California 95131.
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MeSH Terms
Burkitt Lymphoma
Chromomycin A3
Chromosomes, Human
DNA / analysis*
DNA, Neoplasm / analysis
Equipment Design
Flow Cytometry / instrumentation*
Karyotyping / instrumentation,  methods*
Lymphocytes / ultrastructure
Tumor Cells, Cultured
Reg. No./Substance:
0/DNA, Neoplasm; 23491-44-3/Bisbenzimidazole; 7059-24-7/Chromomycin A3; 7440-43-9/Cadmium; 7440-59-7/Helium; 9007-49-2/DNA

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