| Bivariate flow cytometric analysis of p53 and DNA content in hepatocellular carcinoma. | |
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MedLine Citation:
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PMID: 8043159 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bivariate flow cytometric analysis of p53 protein and DNA content was studied in archival specimens of hepatocellular carcinoma (HCC) from Chinese patients and corresponding benign liver tissues from a series of 51 patients at Sun Yat-sen University of Medical Sciences. Extracted nuclei were stained with the fluoresceinated monoclonal antibody PAb 1801, which recognizes human p53 protein (mutant and wild types). The nuclei were counterstained with the DNA stain propidium iodide. They were measured on an Ortho FC-200 flow cytometer and the data acquired and analyzed with an IBM 386 personal computer using Kusuda's Get Simple and List Simple software. Of the 51 hepatomas studied, 26 (51%) were p53 positive as compared with 4 (16%) of 24 samples of benign liver tissue from the same patients (P < .0257). The S-phase fraction of p53-positive HCC (12.3 +/- 8.8%) (SD) was significantly greater (P < .05) than for p53-negative HCC (7.4 +/- 7.2%). p53 Expression did not correlate with age, sex, alpha-fetoprotein, hepatitis B surface antigen, tumor size, tumor grade or survival rate. List Simple software permitted analysis of each specimen together with its isotype control (IgG1) on the same cytogram so that p53 expression could be determined separately for the diploid and aneuploid populations of aneuploid tumors and for tumor cells of diploid tumors in the various phases of the cell cycle. Since p53 (PAb 1801) expression can withstand formalin fixation and pepsin treatment of paraffin-embedded tissues, flow cytometric analysis of archival specimens is feasible, and clinical correlations such as these may be carried out in retrospective studies of other tumors. |
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Authors:
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L Qiao; P Wu; A H Ghaleb; J G Pizzolo; T B Miller; M R Melamed |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology Volume: 16 ISSN: 0884-6812 ISO Abbreviation: Anal. Quant. Cytol. Histol. Publication Date: 1994 Apr |
Date Detail:
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Created Date: 1994-09-01 Completed Date: 1994-09-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8506819 Medline TA: Anal Quant Cytol Histol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 124-30 Citation Subset: IM |
Affiliation:
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Department of Pathology, New York Medical College, Valhalla 10595. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aneuploidy Carcinoma, Hepatocellular / chemistry* DNA, Neoplasm / analysis* Diploidy Female Flow Cytometry / methods* Humans Liver Neoplasms / chemistry* Male Middle Aged Tumor Suppressor Protein p53 / analysis* |
| Grant Support | |
ID/Acronym/Agency:
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R0-1-CA-14134/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Tumor Suppressor Protein p53 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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