Document Detail


Bistability in biochemical signaling models.
MedLine Citation:
PMID:  21954291     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This Teaching Resource provides lecture notes, slides, and a student assignment for a two-part lecture on the principles underlying bistability in biochemical signaling networks, which are illustrated with examples from the literature. The lectures cover analog, or graded, versus digital, all-or-none, responses in cells, with examples from different types of biological processes requiring each. Rate-balance plots are introduced as a method for determining whether generic one-variable systems exhibit one or several stable steady states. Bifurcation diagrams are presented as a more general method for detecting the presence of bistability in biochemical signaling networks. The examples include an artificial toggle switch, the lac operon in bacteria, and the mitogen-activated protein kinase cascade in both Xenopus oocytes and mammalian cells. The second part of the lecture links the concepts of bistability more closely to the mathematical tools provided by dynamical systems analysis. The examples from the first part of the lecture are analyzed with phase-plane techniques and bifurcation analysis, using the scientific programming language MATLAB. Using these programs as a template, the assignment requires the students to implement a model from the literature and analyze the stability of this model's steady states.
Authors:
Eric A Sobie
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-09-20
Journal Detail:
Title:  Science signaling     Volume:  4     ISSN:  1937-9145     ISO Abbreviation:  Sci Signal     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-28     Completed Date:  2012-01-10     Revised Date:  2012-05-02    
Medline Journal Info:
Nlm Unique ID:  101465400     Medline TA:  Sci Signal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  tr10     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and Systems Therapeutics and Systems Biology Center New York, Mount Sinai School of Medicine, New York, NY 10029, USA. eric.sobie@mssm.edu
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MeSH Terms
Descriptor/Qualifier:
Biochemistry / education*,  methods
Computational Biology / education*,  methods
Kinetics
Lac Operon
Mitogen-Activated Protein Kinases / metabolism
Models, Biological*
Signal Transduction / genetics,  physiology*
Grant Support
ID/Acronym/Agency:
P50 GM071558-04/GM/NIGMS NIH HHS; PS0GM071558/GM/NIGMS NIH HHS; T32GM062754/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
EC 2.7.11.24/Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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