Document Detail

Bisphosphonates inhibit 1,25-dihydroxyvitamin D3-induced increase of osteocalcin in plasma of rats in vivo and in culture medium of rat calvaria in vitro.
MedLine Citation:
PMID:  2969277     Owner:  NLM     Status:  MEDLINE    
In order to test whether bisphosphonates, which are potent inhibitors of osteoclastic bone resorption, may also act upon osteoblasts, we studied the effect of dichloromethylenebisphosphonate (Cl2MBP) and 4-amino-1-hydroxybutylidene-1,1-bisphosphonate (AHBuBP) on in vivo levels and in vitro release of osteocalcin, a bone-specific protein produced by osteoblasts. In rats, 161 mumol/kg of Cl2MBP or 1.61 mumol/kg AHBuBP strongly inhibited the increase of plasma osteocalcin induced by 1,25(OH)2D3. The inhibition was measurable within 24 hours after the administration of bisphosphonate and was independent of any change in bone resorption. The effect upon osteocalcin release was also present in calvaria cultures. 250 microM Cl2MBP strongly inhibited the osteocalcin release induced by 10(-8) M 1,25(OH)2D3. In the presence of 1,25(OH)2D3, protein synthesis and DNA synthesis were also decreased, whereas in the absence of 1,25(OH)2D3, protein synthesis was increased. Thus, bisphosphonates affect the production of a bone-specific protein by osteoblasts in addition to their inhibitory action on osteoclasts.
S A Stronski; L Bettschen-Camin; A Wetterwald; R Felix; U Trechsel; H Fleisch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Calcified tissue international     Volume:  42     ISSN:  0171-967X     ISO Abbreviation:  Calcif. Tissue Int.     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-09-02     Completed Date:  1988-09-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905481     Medline TA:  Calcif Tissue Int     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  248-54     Citation Subset:  IM    
Department of Pathophysiology, University of Berne, Switzerland.
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MeSH Terms
Calcitriol / pharmacology*
Calcium-Binding Proteins / blood*
Clodronic Acid / pharmacology*
Culture Techniques
DNA / biosynthesis
Diphosphonates / pharmacology*
Osteoblasts / drug effects,  metabolism,  secretion
Proline / metabolism
Rats, Inbred Strains
Skull / drug effects*,  metabolism
Reg. No./Substance:
0/Calcium-Binding Proteins; 0/Diphosphonates; 104982-03-8/Osteocalcin; 10596-23-3/Clodronic Acid; 147-85-3/Proline; 32222-06-3/Calcitriol; 66376-36-1/Alendronate; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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