| Bisphenol A downregulates CYP19 transcription in JEG-3 cells. | |
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MedLine Citation:
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PMID: 19539015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bisphenol A is an industrial contaminant and is considered to be an endocrine disruptor; its estrogenic property has been reported in many studies. Because of its ubiquitous existence in our environment, bisphenol A has drawn much discussion on its safety issues. Estrogen is important in the maintenance of human pregnancy, and the placenta is the major site of synthesis during this period of time. Aromatase or CYP19 catalyses the conversion of estrogen from its precursor, and is highly expressed in placental cells. In the present study, we examined the ability of the toxicant in suppressing the transcription of CYP19 in JEG-3 cells. Cells treated with bisphenol A displayed a reduced aromatase activity. Real-time PCR analysis indicated that 5muM of the compound significantly reduced the mRNA expression in these cells. As the transcriptional activity of CYP19 gene is controlled by the proximal promoter region of exon I.1 in placental cells, the promoter activity of this gene fragment and exon-I.1-spliced mRNA abundance were also evaluated. Both results indicated that bisphenol A repressed the transcriptional control of promoter I.1. The present study showed that bisphenol A potentially reduced estrogen synthesis by downregulating CYP of placental cells. This information could be useful for evaluating the exposure limit of bisphenol A. |
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Authors:
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Hui Huang; Lai K Leung |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-16 |
Journal Detail:
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Title: Toxicology letters Volume: 189 ISSN: 1879-3169 ISO Abbreviation: Toxicol. Lett. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-07-17 Completed Date: 2009-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 248-52 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aromatase
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biosynthesis*,
genetics Blotting, Western Cell Line Cell Survival / drug effects Endocrine Disruptors / toxicity* Exons / genetics Female Food Contamination Humans Luciferases / genetics Phenols / toxicity* Placenta / cytology, enzymology Pregnancy RNA, Messenger / biosynthesis, genetics Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Endocrine Disruptors; 0/Phenols; 0/RNA, Messenger; 0/Recombinant Proteins; 80-05-7/bisphenol A; EC 1.13.12.-/Luciferases; EC 1.14.14.1/Aromatase |
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