Document Detail

Bisphenol A alters n-6 fatty acid composition and decreases antioxidant enzyme levels in rat testes: a LC-QTOF-based metabolomics study.
MedLine Citation:
PMID:  23024759     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Male reproductive toxicity induced by exposure to bisphenol A (BPA) has been widely reported. The testes have proven to be a major target organ of BPA toxicity, so studying testicular metabolite variation holds promise for the discovery of mechanisms linked to the toxic effects of BPA on reproduction.
METHODOLOGY/PRINCIPAL FINDINGS: Male Sprague-Dawley rats were orally administered doses of BPA at the levels of 0, 50 mg/kg/d for 8 weeks. We used an unbiased liquid chromatography-quadrupole time-of-flight (LC-QTOF)-based metabolomics approach to discover, identify, and analyze the variation of testicular metabolites. Two n-6 fatty acids, linoleic acid (LA) and arachidonic acid (AA) were identified as potential testicular biomarkers. Decreased levels of LA and increased levels of AA as well as AA/LA ratio were observed in the testes of the exposed group. According to these suggestions, testicular antioxidant enzyme levels were detected. Testicular superoxide dismutase (SOD) declined significantly in the exposed group compared with that in the non-exposed group, and the glutathione peroxidase (GSH-Px) as well as catalase (CAT) also showed a decreasing trend in BPA treated group.
CONCLUSIONS/SIGNIFICANCE: BPA caused testicular n-6 fatty acid composition variation and decreased antioxidant enzyme levels. This study emphasizes that metabolomics brings the promise of biomarkers identification for the discovery of mechanisms underlying reproductive toxicity.
Minjian Chen; Bin Xu; Wenliang Ji; Shanlei Qiao; Nan Hu; Yanhui Hu; Wei Wu; Lianglin Qiu; Ruyang Zhang; Yubang Wang; Shoulin Wang; Zuomin Zhou; Yankai Xia; Xinru Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-14
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-01     Completed Date:  2013-02-15     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e44754     Citation Subset:  IM    
State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China.
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MeSH Terms
Benzhydryl Compounds / administration & dosage,  chemistry,  pharmacology*,  urine
Biological Markers / metabolism
Catalase / metabolism
Fatty Acids, Omega-6 / metabolism*
Fatty Acids, Unsaturated / metabolism
Free Radical Scavengers / administration & dosage,  chemistry,  pharmacology*
Glucuronides / urine
Glutathione Peroxidase / metabolism
Metabolomics / methods
Oxidoreductases / metabolism*
Phenols / administration & dosage,  chemistry,  pharmacology*,  urine
Rats, Sprague-Dawley
Superoxide Dismutase / metabolism
Testis / drug effects*,  metabolism*
Reg. No./Substance:
0/Benzhydryl Compounds; 0/Biological Markers; 0/Fatty Acids, Omega-6; 0/Fatty Acids, Unsaturated; 0/Free Radical Scavengers; 0/Glucuronides; 0/Phenols; 0/bisphenol A glucuronide; EC 1.-/Oxidoreductases; EC; EC Peroxidase; EC Dismutase; MLT3645I99/bisphenol A

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