Document Detail

Bisdiamine inhibits extracellular matrix formation and cell proliferation of atrioventricular mesenchyme from developing chick heart valves.
MedLine Citation:
PMID:  10194805     Owner:  NLM     Status:  MEDLINE    
Abnormalities of the cushion tissues lead to atrioventricular septal defects (AVSD) and truncus arteriosus (TA). Bisdiamine exposure in the embryo frequently causes AVSD and TA in the newborn chick, mouse, or rat. We studied the effects of bisdiamine on mesenchymal cells grown in aggregate culture isolated from the developing atrioventricular valves of the stage-36 chick embryo. Fibronectin extracellular matrix formation and cell proliferation in the aggregates were assessed in various media. Chick serum stimulated the cells to produce an extracellular matrix and to divide, and the inclusion of bisdiamine inhibited both responses. If we isolated an extracellular matrix from a monolayer of mesenchymal cells and added the sonicated matrix to the medium containing serum and bisdiamine, the matrix incorporated into the aggregates and the cells entered the mitotic cycle. Our previous work established that cells need to attach to an intact extracellular matrix to begin cell division. Thus, we suggest that bisdiamine inhibits the normal formation of the extracellular matrix, leading to reduced cell proliferation, but it does not affect matrix-cell interaction. The lack of cushion growth in situ may be the cause of AVSD or TA.
M Choy; S L Oltjen; A J Moon; M T Armstrong; P B Armstrong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Teratology     Volume:  59     ISSN:  0040-3709     ISO Abbreviation:  Teratology     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-07-08     Completed Date:  1999-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0153257     Medline TA:  Teratology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  148-55     Citation Subset:  IM    
Division of Pediatric Cardiology, University of California Davis Medical Center, Sacramento 95817, USA.
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MeSH Terms
Cell Division / drug effects
Chick Embryo
Culture Media
Diamines / pharmacology*
Extracellular Matrix / drug effects*,  metabolism
Heart Valves / drug effects,  embryology*
Mesoderm / cytology,  drug effects,  metabolism
Reg. No./Substance:
0/Culture Media; 0/Diamines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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