Document Detail

Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress.
MedLine Citation:
PMID:  20349918     Owner:  NLM     Status:  MEDLINE    
Soluble melanoidins from biscuits were enzymatically solubilized and isolated by sequential ultrafiltration and separated by molecular mass in three different fractions, below 3 kDa, between 3 and 10 kDa, and over 10 kDa; the latter was subsequently digested by simulating gastric plus pancreatic digestive conditions. The four fractions were investigated for their protective effect against an oxidative challenge in HepG2 cells. Pretreatment of cells for 20 h with 0.5-10 microg/mL of any of the four fractions prevented the increased cell damage evoked by the challenge but, except for the intermediate size fraction, did not suppress the increased reactive oxygen species. Antioxidant defenses were rapidly restored after the challenge, and the increase of the oxidative stress biomarker malondialdehyde was prevented by the pretreatment with all but the undigested high molecular mass fraction. The results show that treatment of HepG2 cells with concentrations of biscuit melanoidins within the expected physiological range confers on the cells a significant protection against an oxidative challenge.
María Angeles Martín; Sonia Ramos; Raquel Mateos; José Angel Rufián-Henares; Francisco José Morales; Laura Bravo; Luis Goya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  57     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2010-03-30     Completed Date:  2010-07-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7250-8     Citation Subset:  IM    
Consejo Superior de Investigaciones Cientificas, Instituto del Frio - ICTAN, Jose Antonio Novais 10, Madrid, Spain.
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MeSH Terms
Bread / analysis*
Hep G2 Cells
Molecular Weight
Oxidative Stress / drug effects*
Polymers / chemistry,  pharmacology*
Protective Agents / chemistry,  pharmacology*
Reg. No./Substance:
0/Polymers; 0/Protective Agents; 0/melanoidin polymers

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