Document Detail

Birthdates and number of neurons in the serotonergic raphe nuclei in the rat with prenatal protein malnutrition.
MedLine Citation:
PMID:  12509068     Owner:  NLM     Status:  MEDLINE    
The effect of prenatal protein deprivation on timing of neurogenesis and on number of neurons generated in the serotonergic dorsal (DR) and median raphe (MR) nuclei of the rat was studied. These neurons are of interest because their neurogenesis occurs during the period of malnutrition and their axonal projections participate in the earliest stages of brain development. In this study, dams were maintained on a 25% casein diet or a 6% casein diet 5 weeks prior to mating and throughout pregnancy. At birth, all pups were cross-fostered to dams on a 25% casein diet. Bromodeoxyuridine, a thymidine analog that is incorporated into nuclear deoxyribonucleic acid during the cell cycle synthetic phase, was used as a marker of neurogenesis. Bromodeoxyuridine was administered on either embryonic day 11, 12, 13 or 14. On postnatal day 30, serial sections of raphe nuclei were processed with bromodeoxyuridine immunocytochemistry to determine the number of raphe cells generated on each day and with Nissl stain to determine the total number of cells generated. There were no significant differences between the two diet groups in timing of generation or in total number of cells generated, indicating that neurogenesis of these early generated neurons appears unaffected by concomitant protein deprivation.
R S King; T L Kemper; W A DeBassio; M Ramzan; G J Blatt; D L Rosene; J R Galler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nutritional neuroscience     Volume:  5     ISSN:  1028-415X     ISO Abbreviation:  Nutr Neurosci     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2003-01-01     Completed Date:  2003-03-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100892202     Medline TA:  Nutr Neurosci     Country:  England    
Other Details:
Languages:  eng     Pagination:  391-7     Citation Subset:  IM    
Department of Anatomy and Neurobiology, Boston University School of Medicine, 80 E, Concord Street, Boston, MA 02118, USA.
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MeSH Terms
Brain / embryology,  growth & development
Bromodeoxyuridine / administration & dosage,  analysis
Caseins / administration & dosage
Cell Count
Cell Division
DNA / biosynthesis
Neurons / cytology*
Prenatal Exposure Delayed Effects*
Protein Deficiency*
Raphe Nuclei / cytology*
Rats, Sprague-Dawley
Grant Support
Reg. No./Substance:
0/Caseins; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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