Document Detail


Birth-related expression of c-fos, c-jun and substance P mRNAs in the rat brainstem and pia mater: possible relationship to changes in central chemosensitivity.
MedLine Citation:
PMID:  9878771     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In situ hybridization was used to characterize respiration-related areas of the brainstem activated around the time of birth as well as their postnatal sensitivity to CO2. Levels of mRNA corresponding to the immediate early genes (IEG), c-fos and c-jun, and of substance P precursor, ppt-A, were determined in rat fetuses (E21) and neonatal pups (1 h, 1 day and 6 days after normal birth) and after exposure to hypercapnia (12% CO2 for 1 h). Transient increases in c-fos mRNA were observed in the central chemoreceptor area of the ventral medullary surface (VMS), in the lateral reticular nucleus (LRN), in the nucleus of the solitary tract (NTS), and in the nucleus raphé pallidus (RPA) 1 h after birth. Increased expression of c-fos mRNA in the VMS could also be evoked by hypercapnia and this response was particularly pronounced 1 day after birth. On the other hand, c-jun mRNA could be detected already at E21 in the hypoglossal nucleus (XII) and LRN and these levels were not significantly altered at 1 h after birth. There was, however, an increase in the expression of c-jun mRNA in the pia mater surrounding the brainstem after birth. At 1 day after birth, c-jun mRNA levels had decreased in the LRN and pia mater, and later on (6 days after birth) in XII. Furthermore, the ppt-A mRNA level in NTS increased immediately after birth and remained high 1 and 6 days later. These results suggest that (a) the central chemoreceptor area of the VMS, as well as the NTS, LRN, RPA and pia mater are activated following birth; (b) the VMS, but not the other structures examined, can be activated immediately after birth by hypercapnia; and (c) increased expression of ppt-A mRNA may be related to the transition of respiratory control at birth.
Authors:
H R Wickström; H Holgert; T Hökfelt; H Lagercrantz
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Developmental brain research     Volume:  112     ISSN:  0165-3806     ISO Abbreviation:  Brain Res. Dev. Brain Res.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-04-13     Completed Date:  1999-04-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8908639     Medline TA:  Brain Res Dev Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  255-66     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Elsevier Science B.V.
Affiliation:
Department of Women and Child Health, Karolinska Institute, S-171 77, Stockholm, Sweden. ronny.wickstrom@kbh.ki.se
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn / growth & development,  metabolism
Brain / embryology*,  metabolism*,  physiology
Brain Stem / embryology,  metabolism
Chemoreceptor Cells / physiology
Delivery, Obstetric*
Embryonic and Fetal Development / physiology
Female
Genes, Immediate-Early / genetics*
Male
Medulla Oblongata / metabolism
Pia Mater / embryology,  metabolism
Protein Precursors / genetics
Proto-Oncogene Proteins c-fos / genetics
Proto-Oncogene Proteins c-jun / genetics
RNA, Messenger / metabolism*
Rats / embryology
Rats, Sprague-Dawley
Substance P / genetics*
Tachykinins / genetics
Chemical
Reg. No./Substance:
0/Protein Precursors; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/Tachykinins; 0/preprotachykinin; 33507-63-0/Substance P

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