Document Detail


Birth of neural progenitors during the embryonic period of sexual differentiation in the Japanese quail brain.
MedLine Citation:
PMID:  22628012     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several brain areas in the diencephalon are involved in the activation and expression of sexual behavior, including in quail the medial preoptic nucleus (POM). However, the ontogeny of these diencephalic brain nuclei has not to this date been examined in detail. We investigated the ontogeny of POM and other steroid-sensitive brain regions by injecting quail eggs with 5-bromo-2-deoxyuridine (BrdU) at various stages between embryonic day (E)3 and E16 and killing animals at postnatal (PN) days 3 or 56. In the POM, large numbers of BrdU-positive cells were observed in subjects injected from E3-E10, the numbers of these cells was intermediate in birds injected on E12, and most cells were postmitotic in both sexes on E14-E16. Injections on E3-E4 labeled large numbers of Hu-positive cells in POM. In contrast, injections performed at a later stage labeled cells that do not express aromatase nor neuronal markers such as Hu or NeuN in the POM and other steroid-sensitive nuclei and thus do not have a neuronal phenotype in these locations, contrary to what is observed in the telencephalon and cerebellum. No evidence could also be collected to demonstrate that these cells have a glial nature. Converging data, including the facts that these cells divide in the brain mantle and express proliferating cell nuclear antigen (PCNA), a cell cycling marker, indicate that cells labeled by BrdU during the second half of embryonic life are slow-cycling progenitors born and residing in the brain mantle. Future research should now identify their functional significance.
Authors:
Sylvia M Bardet; Karen Mouriec; Jacques Balthazart
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  520     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-22     Completed Date:  2013-04-18     Revised Date:  2013-12-18    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4226-53     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Brain* / cytology,  embryology,  growth & development
Bromodeoxyuridine / metabolism
Cell Proliferation
Coturnix
Embryo, Mammalian
Embryo, Nonmammalian
Female
Male
Nerve Tissue Proteins / metabolism
Neural Stem Cells / physiology*
Neurogenesis / physiology*
Proliferating Cell Nuclear Antigen / metabolism
Sex Differentiation*
Grant Support
ID/Acronym/Agency:
R01 MH050388/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Proliferating Cell Nuclear Antigen; G34N38R2N1/Bromodeoxyuridine
Comments/Corrections

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