|Birth of live mice derived by in vitro fertilization with spermatozoa retrieved up to twenty-four hours after death.|
|PMID: 9433804 Owner: NLM Status: MEDLINE|
|The objective of this study was to determine the viability and fertility of mouse spermatozoa obtained at various postmortem intervals. Male mice were euthanized, and the bodies were kept at approximately 22 degrees C for up to 24 hr. The epididymides were removed and spermatozoa were allowed to swim out into Minimum Essential Medium supplemented with bovine serum albumin. Motility of spermatozoa retrieved within 12 hr postmortem was approximately 60%, whereas motility of those obtained 6 to 12 hr later decreased significantly (P < 0.05). There appeared to be no differences in the percentages of spermatozoa with intact plasma and acrosomal membranes regardless of the time after death. After in vitro fertilization of oocytes with spermatozoa collected immediately after death or at 6, 12, 18, or 24 hr postmortem, the cleavage rates were 81%, 70%, 64%, 34%, and 19%, respectively. Once oocytes were fertilized, more than 65% developed into morulae/blastocysts. Transfer of a total of 166 embryos produced in vitro with postmortem spermatozoa resulted in the birth of 44 live pups (26.5%). Of these 44, 3 live mice were derived by transfer of 11 embryos (27.3%) produced with 24-hr postmortem spermatozoa. Histological examination of the testes and epididymides obtained at various postmortem intervals revealed that degenerative changes of the testes occurred within 6 hr, whereas those of the epididymides were less obvious until 6 hr later. These changes included pyknosis, release of intracellular contents, and disruption of intercellular bridges of the germ cells. This study has demonstrated that spermatozoa recovered from a dead animal as long as 24 hr after death can be used to fertilize oocytes, and that the resulting zygotes can develop into live young.|
|N Songsasen; J Tong; S P Leibo|
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|Type: Journal Article; Research Support, Non-U.S. Gov't|
|Title: The Journal of experimental zoology Volume: 280 ISSN: 0022-104X ISO Abbreviation: J. Exp. Zool. Publication Date: 1998 Feb|
|Created Date: 1998-02-04 Completed Date: 1998-02-04 Revised Date: 2006-11-15|
Medline Journal Info:
|Nlm Unique ID: 0375365 Medline TA: J Exp Zool Country: UNITED STATES|
|Languages: eng Pagination: 189-96 Citation Subset: IM|
|Department of Biomedical Sciences, University of Guelph, Ontario, Canada.|
|APA/MLA Format Download EndNote Download BibTex|
Blastocyst / cytology, physiology
Epididymis / pathology
Fertilization in Vitro*
Mice, Inbred Strains
Morula / cytology, physiology
Oocytes / cytology, physiology*
Serum Albumin, Bovine
Spermatozoa / cytology*, physiology*
Testis / pathology
Zygote / cytology, physiology
|0/Serum Albumin, Bovine|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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