Document Detail


Birth after double cryopreservation of human oocytes at metaphase II and pronuclear stages.
MedLine Citation:
PMID:  16500354     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To document the possibility to double-cryopreserve human metaphase II and pronuclear oocytes. DESIGN: Case report. SETTING: University-based IVF center. PATIENT(S): A couple with primary andrological infertility. INTERVENTION(S): Pronuclear stage oocytes derived after sperm injection into frozen-thawed metaphase II oocytes were cryopreserved again for later transfer. MAIN OUTCOME MEASURE(S): Post-thaw survival rates and pregnancy. RESULT(S): Five metaphase II oocytes survived the freeze-thaw procedure. Three of five oocytes presented with two pronuclei after sperm injection and were cryopreserved again. Post-thawing, all three oocytes developed into good quality embryos for transfer. A singleton pregnancy was achieved, resulting in the birth of a healthy girl. CONCLUSION(S): Double-cryopreservation of oocytes at metaphase II and at pronuclear stage is a treatment option in human-assisted reproduction.
Authors:
Markus Montag; Katrin van der Ven; Christoph Dorn; Vladimir Isachenko; Eugenia Isachenko; Hans van der Ven
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Fertility and sterility     Volume:  85     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-27     Completed Date:  2006-03-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  751.e5-751.e7     Citation Subset:  IM    
Affiliation:
Department of Gynecological Endocrinology and Reproductive Medicine, University of Bonn, Bonn, Germany. Markus.Montag@ukb.uni-bonn.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Cryopreservation*
Embryo Transfer
Female
Humans
Infant, Newborn
Male
Metaphase*
Oocytes / cytology*
Parturition*
Pregnancy
Prophase*
Sperm Injections, Intracytoplasmic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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