Document Detail

Biotransformation and improved enzymatic extraction of chlorogenic acid from coffee pulp by filamentous fungi.
MedLine Citation:
PMID:  23341203     Owner:  NLM     Status:  Publisher    
The highest enzymatic extraction of covalent linked chlorogenic (36.1%) and caffeic (33%) acids from coffee pulp was achieved by solid-state fermentation with a mixture of three enzymatic extracts produced by Aspergillus tamarii, Rhizomucor pusillus and Trametes sp. Enzyme extracts were produced in a practical inexpensive way. Synergistic effects on the extraction yield were observed when more than one enzyme extract was used. In addition, biotransformation of chlorogenic acid by A. niger C23308 was studied. Equimolar transformation of chlorogenic acid into caffeic and quinic acids was observed during the first 36 h in submerged culture. Subsequently, after 36 h, equimolar transformation of caffeic acid into protocatechuic acid was observed; this pathway is being reported for the first time for A. niger. Quinic acid was used as a carbon source by A. niger C23308. This study presents the potential of using coffee pulp to produce enzymes and compounds such as chlorogenic acid with biological activities. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 2013.
María Teresa Torres-Mancera; Itzamná Baqueiro-Peña; Arturo Figueroa-Montero; Gabriela Rodríguez-Serrano; Eduardo González-Zamora; Ernesto Favela-Torres; Gerardo Saucedo-Castañeda
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-22
Journal Detail:
Title:  Biotechnology progress     Volume:  -     ISSN:  1520-6033     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 American Institute of Chemical Engineers (AIChE).
Dept. of Biotechnology, Metropolitan Autonomous University, Iztapalapa Campus. Av. San Rafael Atlixco 186, Col. Vicentina, C.P. 09340, D.F. Mexico.
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