| Biotransformation of extracted digitoxin from Digitalis lanata by Streptomyces. | |
| | |
MedLine Citation:
|
PMID: 21699088 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
The biotransformation of digitoxin and some of its derivatives extracted from Digitalis lanata by Streptomyces isolated species was investigated. Cultures of a Streptomyces strain designated EUSA2003B, isolated from an Egyptian soil sample, efficiently induced selective 12beta-hydroxylation of the steroid aglycone of digitoxin (DT) and its alpha-acetyl and beta-methyl derivatives. The transformation reaction was performed within a 5-day fermentation process, products were isolated and their aglycone moiety was obtained by acid hydrolysis and their structures were elucidated by 13C and 1H NMR. The biotransformation resulted mainly digoxin (DG, approximately 87%), meanwhile, digoxigenone (DGON, approximately 7.0%) was also afforded as a side product. The present study revealed that: 1-Streptomyces isolate EUSA2003B harbors its specific 12beta-hydroxlase and has the capability to transform DT and it's alpha-acetyl and beta-methyl derivatives into their corresponding digoxins at reasonable yields. 2-The minor structural differences in the trisaccharide side chain seemed ineffective on the transformational capability of this organism. 3-The Streptomyces might also possess a specific glycosidase that splits the saccharidic side chain beside another dehydrogenase that oxidizes C3 at the steroid nucleus into its ketone form (DGON). |
| | |
Authors:
|
S Keshk; M Mostafa; F Tawfik; I Elshemy |
Related Documents
:
|
22568728 - Cobalt- versus ruthenium-catalyzed alder-ene reaction for the synthesis of credneramide... 23151918 - Investigation of the biological properties of (hetero)aromatic thiosemicarbazones. 23625908 - 1,1-diphenyl,2-picrylhydrazyl free radical scavenging, bactericidal, fungicidal and lei... 12663658 - Autocatalytic oscillations in the early phase of the photoreduced methyl viologen-initi... 19606898 - Synthesis and applications of biomedical and pharmaceutical polymers via click chemistr... 6498578 - Comparative effects of volatile anaesthetic agents and nitrous oxide on human leucocyte... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Die Pharmazie Volume: 66 ISSN: 0031-7144 ISO Abbreviation: Pharmazie Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-06-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9800766 Medline TA: Pharmazie Country: Germany |
Other Details:
|
Languages: eng Pagination: 458-62 Citation Subset: IM |
Affiliation:
|
Chemistry Department, King Khalid University Abha, The Kingdom of Saudi Arabia. sherifkeshk@yahoo.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation of two new bioactive proanthocyanidins from Cistus salvifolius herb extract.
Next Document: Skin penetration of silica microparticles.