Document Detail


Biotransformation and elimination of 1-nitropyrene in the isolated perfused lung: effects of pretreating rats with phenobarbitone, beta-naphthoflavone, benz(A)anthracene or their mixtures.
MedLine Citation:
PMID:  9772702     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The isolated perfused rat lung (IPL) was perfused with 60 ml of recirculating Krebs-Ringer solution containing 150 micrograms of 1-nitropyrene (1-NP) for 1 h. The 1-NP was administered to the IPL by the intratracheal or intravascular route. At specific time points after 1-NP administration, perfusate samples were removed from the IPL and analysed for 1-NP and its metabolites by HPLC. Monohydroxynitropyrenes, dihydroxynitropyrenes and 1-NP were found to be present in the perfusate. The time course of 1-NP concentrations in the perfusate could be described by a one-compartment pharmacokinetic model. Pretreatment of rats with beta-naphthoflavone (BNF), benz(a) anthracene (BA) or a mixture of phenobarbitone (PB) and BNF (PB + BNF) significantly enhanced the metabolism of 1-NP and decreased the mean residence time (MRT) of 1-NP in the perfusate. Pretreatment of rats with these mixed-function oxidase inducers also increased significantly the absorption of 1-NP by the lung when it was administered intratracheally. In contrast, pretreatment of rats with PB did not appear to have any effect on the pharmacokinetics of 1-NP in the IPL.
Authors:
F C Law; S X He; Y C Chui
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Yao xue xue bao = Acta pharmaceutica Sinica     Volume:  31     ISSN:  0513-4870     ISO Abbreviation:  Yao Xue Xue Bao     Publication Date:  1996  
Date Detail:
Created Date:  1998-11-12     Completed Date:  1998-11-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  21710340R     Medline TA:  Yao Xue Xue Bao     Country:  CHINA    
Other Details:
Languages:  eng     Pagination:  568-76     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benz(a)Anthracenes / pharmacology
Biotransformation
Enzyme Inhibitors / pharmacology
Lung / metabolism*
Male
Mutagens / pharmacokinetics*
Perfusion
Phenobarbital / pharmacology
Pyrenes / pharmacokinetics*
Rats
Rats, Wistar
beta-Naphthoflavone / pharmacology
Chemical
Reg. No./Substance:
0/Benz(a)Anthracenes; 0/Enzyme Inhibitors; 0/Mutagens; 0/Pyrenes; 50-06-6/Phenobarbital; 5522-43-0/1-nitropyrene; 56-55-3/benz(a)anthracene; 6051-87-2/beta-Naphthoflavone

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