Document Detail

Biotransformation of D-methionine into L-methionine in the cascade of four enzymes.
MedLine Citation:
PMID:  17534960     Owner:  NLM     Status:  MEDLINE    
D-Methionine was converted to L-methionine in a reaction system where four enzymes were used. D-amino acid oxidase (D-AAO) from Arthrobacter protophormiae was used for the complete conversion of D-methionine to 2-oxo-4-methylthiobutyric acid. Catalase was added to prevent 2-oxo-4-methylthiobutyric acid decarboxylation. In the second reaction step, L-phenylalanine dehydrogenase (L-PheDH) from Rhodococcus sp. was used to convert 2- oxo-4-methylthiobutyric acid to L-methionine, and formate dehydrogenase (FDH) from Candida boidinii was added for NADH regeneration. Enzyme kinetics of all enzymes was analyzed in detail. Mathematical models for separate reactions steps, as well as for the complete system were developed and validated in the batch reactor experiments. Complete conversion of D-methionine to L-methionine was achieved. Considering that both enzymes act on different substrates, such a system could be easily employed for the synthesis of other amino acids from D-isomer, as well as from the racemate of a certain amino acid (DL-amino acid).
Z Findrik; D Vasić-Racki
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  98     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-10-24     Completed Date:  2008-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  956-67     Citation Subset:  IM    
Faculty of Chemical Engineering and Technology, University of Zagreb, Savska c. 16, HR-10 000 Zagreb, Croatia.
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MeSH Terms
Amino Acid Oxidoreductases / chemistry*,  metabolism
Arthrobacter / enzymology
Biotechnology / methods
Candida / enzymology
Catalase / chemistry*,  metabolism
D-Amino-Acid Oxidase / chemistry*,  metabolism
Formate Dehydrogenases / chemistry*,  metabolism
Hydrogen-Ion Concentration
Methionine / analogs & derivatives,  chemistry*,  metabolism
Models, Chemical
NAD / chemistry,  metabolism
Rhodococcus / enzymology
Reg. No./Substance:
53-84-9/NAD; 583-92-6/2-keto-4-methylthiobutyric acid; 63-68-3/Methionine; EC; EC Dehydrogenases; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.3.-/phenylalanine oxidase; EC Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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