Document Detail


Biotransformation of 2,2',5,5'-tetrachlorobiphenyl (PCB 52) and 3,3',4,4'-tetrachlorobiphenyl (PCB 77) by liver microsomes from four species of sea turtles.
MedLine Citation:
PMID:  21480586     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The rates of oxidative metabolism of two tetrachlorobiphenyl congeners were determined in hepatic microsomes from four species of sea turtles, green (Chelonia mydas), olive ridley (Lepidochelys olivacea), loggerhead (Caretta caretta), and hawksbill (Eretmochelys imbricata). Hydroxylation of 3,3',4,4'-tetrachlorobiphenyl (PCB 77), an ortho-meta unsubstituted rodent CYP1A substrate PCB, was not observed in sea turtle microsomes. Sea turtle microsomes hydroxylated 2,2',5,5'-tetrachlorobiphenyl (PCB 52), a meta-para unsubstituted rodent CYP2 substrate PCB, at rates ranging from less than 0.5 to 53 pmol/min/mg protein. The cytochrome P450 (CYP) inhibitor ketoconazole inhibited hydroxylation of PCB 52, supporting the role of CYP catalysis. Sea turtle PCB 52 hydroxlyation rates strongly correlated with immunodetected CYP2-like and less so with CYP3-like hepatic proteins. Testosterone 6β-, 16α-, 16β- hydroxylase activities were also significantly correlated with the expression of these isoforms, indicating that CYP2 or CYP3 proteins are responsible for PCB hydroxylation in sea turtles. This study indicated species-specific PCB biotransformation in sea turtles and preferential elimination of meta-para unsubstituted PCB congeners over ortho-meta unsubstituted PCB congeners consistent with PCB accumulation patterns observed in tissues of sea turtles.
Authors:
Kristine Richardson; Daniel Schlenk
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-11
Journal Detail:
Title:  Chemical research in toxicology     Volume:  -     ISSN:  1520-5010     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807448     Medline TA:  Chem Res Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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