Document Detail


Biotin determination in food supplements by an electrochemical magneto biosensor.
MedLine Citation:
PMID:  22841112     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
An electrochemical magneto biosensor for the rapid determination of biotin in food samples is reported. The affinity reaction was performed on streptavidin-modified magnetic microbeads as a solid support in a direct competitive format. The biotinylated horseradish peroxidase enzyme (biotin-HRP) competes with free biotin in the sample for the binding sites of streptavidin on the magnetic microbeads. The modified magnetic beads were then easily captured by a magneto graphite-epoxy composite electrode and the electrochemical signal was based on the enzymatic activity of the HRP enzyme under the addition of H(2)O(2) as the substrate and o-phenilendiamine as cosubstrate. The response was electrochemically detected by square wave voltammetry. The limit of detection was 8.4×10(-8)molL(--1) of biotin (20μgL(--1)) with a dynamic range from 0.94 to 2.4×10(-7)molL(--1). Biotin-fortified commercial dietary supplement and infant formula samples were evaluated obtaining good performances in the results. Total time of analysis was 40min per 20 assays.
Authors:
Silvina V Kergaravat; Gabriel A Gómez; Silvia N Fabiano; Tamara I Laube Chávez; María I Pividori; Silvia R Hernández
Publication Detail:
Type:  Journal Article     Date:  2012-05-11
Journal Detail:
Title:  Talanta     Volume:  97     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  484-90     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Laboratorio de Sensores y Biosensores, Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina.
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