Document Detail


Biotin assessment in foods and body fluids by a protein binding assay (PBA).
MedLine Citation:
PMID:  2722428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The biotin content of plant and animal food samples as well as of human plasma and urine specimens was assessed by a modified protein binding assay (PBA). Protein bound biotin in samples was released by digestion with papain, food samples were in addition treated previously with liquid nitrogen. Comparing analyses by use of the conventional microbiological test with L. plantarum resulted in slightly enhanced biotin values, in case of plasma by 9.2% and in case of urine by 10.3%, but the results obtained by both methods correlated well (r = 0.926 for plasma and 0.934 for urine). Regarding food analysis the additional comparison of different disintegration procedures led to best reproducible results by using the PBA with preceding papain digestion.
Authors:
R Bitsch; I Salz; D Hötzel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition     Volume:  59     ISSN:  0300-9831     ISO Abbreviation:  Int J Vitam Nutr Res     Publication Date:  1989  
Date Detail:
Created Date:  1989-07-07     Completed Date:  1989-07-07     Revised Date:  2007-02-21    
Medline Journal Info:
Nlm Unique ID:  1273304     Medline TA:  Int J Vitam Nutr Res     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  59-64     Citation Subset:  IM    
Affiliation:
Haushalts-und Ernährungswissenschaft im FB 6, Universität-GH-Paderborn, F.R.G.
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MeSH Terms
Descriptor/Qualifier:
Adult
Avidin / metabolism
Binding, Competitive
Biotin / analysis*,  blood,  urine
Female
Food Analysis / methods*
Humans
Male
Protein Binding
Radioimmunoassay
Chemical
Reg. No./Substance:
1405-69-2/Avidin; 58-85-5/Biotin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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