Document Detail


Biotin requirements are lower in human Jurkat lymphoid cells but homeostatic mechanisms are similar to those of HepG2 liver cells.
MedLine Citation:
PMID:  20357078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The following proteins are candidates for maintaining biotin homeostasis in humans: the biotin transporters sodium-dependent multivitamin transporter (SMVT) and monocarboxylate transporter 1, the biotinyl-protein ligase holocarboxylase synthetase (HCS), and the lysine-epsilon-biotin hydrolase biotinidase. Liver cells are supplied through the portal vein with high levels of water-soluble vitamins compared with those of peripheral tissues. We hypothesized that the mechanisms of biotin homeostasis are qualitatively and quantitatively different in cells derived from human liver (HepG2 cells) and lymphoid tissues (Jurkat cells). Cells were cultured in biotin-defined media, representing deficient (D), normal (N), and supplemented (S) individuals. Biotinylation of carboxylases depended on biotin availability in both cell types, but HepG2 cells required 3 times more biotin than Jurkat cells to maintain normal levels of holocarboxylases. The expression of biotin transporters was less in both types in medium S compared with cells in media D and N; in contrast, the expression of HCS was higher in cells in medium S compared with the other cells. The abundance of 3-methylcrotonyl-CoA carboxylase mRNA was lower in cells in medium D than cells in media N and S. The enrichment of biotinylated histones was higher at the SMVT promoter 1 in HepG2 and Jurkat cells in medium S compared with the corresponding cells in media D and N, presumably repressing the SMVT gene. The mechanisms of biotin homeostasis are qualitatively similar but quantitatively different in HepG2 and Jurkat cells; HCS, histone biotinylation, and biotin transporters play a role in homeostasis in both.
Authors:
Gaganpreet Kaur Mall; Yap Ching Chew; Janos Zempleni
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-03-31
Journal Detail:
Title:  The Journal of nutrition     Volume:  140     ISSN:  1541-6100     ISO Abbreviation:  J. Nutr.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-21     Completed Date:  2010-06-17     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1086-92     Citation Subset:  IM    
Affiliation:
Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA.
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MeSH Terms
Descriptor/Qualifier:
Biotin / metabolism*
Biotinylation
Carboxy-Lyases / metabolism
Culture Media
Hep G2 Cells
Hepatocytes / metabolism*
Histones / metabolism
Homeostasis / physiology*
Humans
Jurkat Cells
Lymphocytes / metabolism*
Grant Support
ID/Acronym/Agency:
DK063945/DK/NIDDK NIH HHS; DK077816/DK/NIDDK NIH HHS; DK082476/DK/NIDDK NIH HHS; ES015206/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Histones; 58-85-5/Biotin; EC 4.1.1.-/Carboxy-Lyases
Comments/Corrections

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