| Biotic elicitation of the Trifolium pratense L. suspension culture. | |
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MedLine Citation:
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PMID: 18683426 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The important substances of secondary metabolism include flavonoids and isoflavonoids. The Trifolium pratense L. (Fabaceae) suspension culture's yield of these metabolites is low, thus an attempt was made to increase the production by elicitation. The endogenous signal substances of the plants' defensive responses include jasmonic acid that also functions as a biotic elicitor in the case of exogenous application. In the experiment the authors monitored the impact of 4 different concentrations of jasmonic acid on the Trifolium pratense L. (variety DO-8 and variety DO-9) suspension culture's yield. The culture was cultivated in Gamborg medium to which 2 mg.l(-1) of 2,4-dichlorophenoxyacetic acid and 2 mg.l(-1) of 6-benzylaminopurine were added. The maximum increase in the production of flavonoids was achieved, when compared with the control samples, with both varieties of the 500 micromol concentration (DO-9, 6-hour application by 140%; DO-8, 24-hour application by 65%). The production of isoflavonoids (genistin, daidzein, genistein, and formononetine) was best stimulated in both varieties by the 50 micromol concentration (in the case of DO-9 variety, the 48-hour application increased the content of genistin by up to 845%). |
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Authors:
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M Kasparová; T Siatka; J Dusek |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Ceská a Slovenská farmacie : casopis Ceské farmaceutické spolecnosti a Slovenské farmaceutické spolecnosti Volume: 57 ISSN: 1210-7816 ISO Abbreviation: Ceska Slov Farm Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-08-07 Completed Date: 2008-09-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9433765 Medline TA: Ceska Slov Farm Country: Czech Republic |
Other Details:
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Languages: eng Pagination: 107-10 Citation Subset: IM |
Affiliation:
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Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy Heyrovského 1203, 500 05 Hradec Králové, Czech Republic. kasparova@faf.cuni.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Cyclopentanes / pharmacology* Flavonoids / biosynthesis* Isoflavones / biosynthesis Oxylipins / pharmacology* Plant Growth Regulators / pharmacology Trifolium / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cyclopentanes; 0/Flavonoids; 0/Isoflavones; 0/Oxylipins; 0/Plant Growth Regulators; 6894-38-8/jasmonic acid |
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