Document Detail

Biosynthetic characterization and biochemical features of the third natural nisin variant, nisin Q, produced by Lactococcus lactis 61-14.
MedLine Citation:
PMID:  19120645     Owner:  NLM     Status:  MEDLINE    
AIMS: To characterize the genetic and biochemical features of nisin Q. METHODS AND RESULTS: The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster were identified. Nisin Q production was confirmed from the nisQ-introduced nisin Z producer. In the reporter assay, nisin Q exhibited an induction level that was threefold lower than that of nisin A. Nisin Q demonstrated an antimicrobial spectrum similar to those of the other nisins. Under oxidizing conditions, nisin Q retained a higher level of activity than nisin A. This higher oxidative tolerance could be attributed to the presence of only one methionine residue in nisin Q, in contrast to other nisins that contain two. CONCLUSIONS: The 11 orfs of the nisin producers were identical with regard to their functions. The antimicrobial spectra of the three natural nisins were similar. Nisin Q demonstrated higher oxidative tolerance than nisin A. SIGNIFICANCE AND IMPACT OF THE STUDY: Genetic and biochemical features of nisin Q are similar to those of other variants. Moreover, owing to its higher oxidative tolerance, nisin Q is a potential alternative for nisin A.
F Yoneyama; M Fukao; T Zendo; J Nakayama; K Sonomoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  105     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-01-05     Completed Date:  2009-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1982-90     Citation Subset:  IM    
Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Higashi-ku, Fukuoka, Japan.
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MeSH Terms
Anti-Bacterial Agents / biosynthesis*,  pharmacology
Bacteria / drug effects
Bacteriocins / genetics*,  metabolism,  pharmacology
DNA, Bacterial / genetics
Lactococcus lactis / genetics*,  metabolism
Microbial Sensitivity Tests
Molecular Sequence Data
Multigene Family / genetics
Nisin / analogs & derivatives*,  biosynthesis,  genetics,  pharmacology
Open Reading Frames
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacteriocins; 0/DNA, Bacterial; 1414-45-5/Nisin

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