| Biosynthesis of salicylic acid in fungus elicited Catharanthus roseus cells. | |
| | |
MedLine Citation:
|
PMID: 19251288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Feeding experiments using [1-(13)C]-d-glucose to Catharanthus roseus (L.) G.Don cell suspension cultures followed by elicitation with Pythium aphanidermatum extract were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. A strongly labeled C-7 and a symmetrical partitioning of the label between C-2 and C-6 would occur if SA was synthesized from phenylalanine. In case of the isochorismate pathway, a relatively lower incorporation at C-7 and a non-symmetrical incorporation at C-2 and C-6 would be obtained. Relatively, high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by (13)C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds. However, different enrichment ratios of the labeled carbons in SA and 2,3-DHBA indicate the use of different isochorismate pools, which means that their biosynthesis is separated in time and/or space. |
| | |
Authors:
|
Natali R Mustafa; Hye Kyong Kim; Young Hae Choi; Cornelis Erkelens; Alfons W M Lefeber; Gerwin Spijksma; Robert van der Heijden; Robert Verpoorte |
Related Documents
:
|
22193928 - Admission uric acid levels and length of expectant management in preterm preeclampsia. 10592528 - On-line and off-line monitoring of the production of cephalosporin c by acremonium chry... 8509338 - Biosynthesis and stereochemistry of the autoinducer controlling luminescence in vibrio ... 15012188 - Nonphotosynthetic metabolism in plastids. 11738238 - Detection of vitamin k-dependent proteins in venoms with a monoclonal antibody specific... 667088 - Role of phosphatidylcholine on microsomal chain elongation and the fate of stearoyl-coa... |
Publication Detail:
|
Type: Journal Article Date: 2009-02-27 |
Journal Detail:
|
Title: Phytochemistry Volume: 70 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 2009 Mar |
Date Detail:
|
Created Date: 2009-03-24 Completed Date: 2009-07-01 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: United States |
Other Details:
|
Languages: eng Pagination: 532-9 Citation Subset: IM |
Affiliation:
|
Division of Pharmacognosy, Leiden University, Einsteinweg 55, Leiden, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carbon Isotopes Catharanthus / metabolism*, microbiology Cells, Cultured Hydroxybenzoic Acids / chemistry, metabolism Nuclear Magnetic Resonance, Biomolecular Pythium / physiology* Salicylic Acid / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Carbon Isotopes; 0/Hydroxybenzoic Acids; 303-38-8/2-pyrocatechuic acid; 69-72-7/Salicylic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cytotoxic sesquiterpenoids from Winteraceae of Caledonian rainforest.
Next Document: Accumulation, speciation, and coordination of arsenic in an inbred line and a wild type cultivar of ...