Document Detail


Biosynthesis of salicylic acid in fungus elicited Catharanthus roseus cells.
MedLine Citation:
PMID:  19251288     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Feeding experiments using [1-(13)C]-d-glucose to Catharanthus roseus (L.) G.Don cell suspension cultures followed by elicitation with Pythium aphanidermatum extract were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. A strongly labeled C-7 and a symmetrical partitioning of the label between C-2 and C-6 would occur if SA was synthesized from phenylalanine. In case of the isochorismate pathway, a relatively lower incorporation at C-7 and a non-symmetrical incorporation at C-2 and C-6 would be obtained. Relatively, high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by (13)C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds. However, different enrichment ratios of the labeled carbons in SA and 2,3-DHBA indicate the use of different isochorismate pools, which means that their biosynthesis is separated in time and/or space.
Authors:
Natali R Mustafa; Hye Kyong Kim; Young Hae Choi; Cornelis Erkelens; Alfons W M Lefeber; Gerwin Spijksma; Robert van der Heijden; Robert Verpoorte
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Publication Detail:
Type:  Journal Article     Date:  2009-02-27
Journal Detail:
Title:  Phytochemistry     Volume:  70     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-24     Completed Date:  2009-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  532-9     Citation Subset:  IM    
Affiliation:
Division of Pharmacognosy, Leiden University, Einsteinweg 55, Leiden, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Carbon Isotopes
Catharanthus / metabolism*,  microbiology
Cells, Cultured
Hydroxybenzoic Acids / chemistry,  metabolism
Nuclear Magnetic Resonance, Biomolecular
Pythium / physiology*
Salicylic Acid / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Hydroxybenzoic Acids; 303-38-8/2-pyrocatechuic acid; 69-72-7/Salicylic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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