Document Detail

Biosynthesis of pyrrolidone carboxylic acid in hairless mouse epidermis.
MedLine Citation:
PMID:  870563     Owner:  NLM     Status:  MEDLINE    
[3H]Glutamic acid (PCA) was followed with time after a single subcutaneous injection. PCA specific activity increased slowly, reaching a peak at 3 to 4 days after injection of the labeled amino acid, after which it slowly decline. Incorporation of [3H]glutamic acid into epidermal PCA was markedly inhibited by a single topical application of cycloheximide. Topical application of cycloheximide 2 hr prior to [3H]glutamate injection caused a significantly greater reduction in PCA specific activity (determined 3 days after injection) than cycloheximide treatment 3 hr after administration of the labeled amino acid. Ninety-seven percent of the PCA content of hairless mouse epidermis was shown to reside in the stratum corneum. These observations indicate the involvement of protein synthesis in the formation of PCA from glutamic acid rather than a direct conversion of the amino acid. The high level of PCA in mammalian epidermis appears to be caused by its accumulation in the stratum corneum accompainied by a relatively slow rate of PCA turnover in comparison to other tissues.
N W DeLapp; D K Dieckman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  68     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-06-30     Completed Date:  1977-06-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  293-8     Citation Subset:  AIM; IM    
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MeSH Terms
Acetone / pharmacology
Cycloheximide / administration & dosage,  pharmacology
DNA / metabolism
Glutamates / metabolism
Mice, Nude
Pyrrolidinones / metabolism*
Pyrrolidonecarboxylic Acid / metabolism*
Skin / metabolism*
Reg. No./Substance:
0/Glutamates; 0/Pyrrolidinones; 66-81-9/Cycloheximide; 67-64-1/Acetone; 9007-49-2/DNA; 98-79-3/Pyrrolidonecarboxylic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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