Document Detail

Biosynthesis of porphyrins in Rhodopseudomonas palustris--VI. The effect of metals, thiols and other reagents on the activity of uroporphyrinogen decarboxylase.
MedLine Citation:
PMID:  3595985     Owner:  NLM     Status:  MEDLINE    
The effect of several metals and reagents on the decarboxylation rate of uroporphyrinogen I by using a 16-fold purified preparation of Uroporphyrinogen Decarboxylase from Rhodopseudomonas palustris, was studied. 1 mM Hg2+ and Cu2+ were strong inhibitors, 1 mM Zn2+ and Fe2+ under certain conditions and 1 mM Fe3+ and Cr3+ also inactivated the enzyme, but Pb2+, Cd2+ and Al3+ did not. Metals inhibition was reversed by 1 mM GSH or CySH. 0.1 mM DTNB and PCMB, 1 mM pyridoxal phosphate and 100 mM chloral hydrate, as well as 1 mM 2-methoxy-5-nitrotropone and 0.2 mM diethylpyrocarbonate inhibited Uroporphyrinogen Decarboxylase; while GSH, CySH, N-ethylmaleimide, sodium thioglycolate, 1,4-dithioerythritol, EDTA and O-phenantroline did not modify activity. Data obtained would indicate that one cysteine, one or two histidine residues and probably a lysine group are required for enzyme activity.
G E Koopmann; A M Batlle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  19     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1987  
Date Detail:
Created Date:  1987-07-29     Completed Date:  1987-07-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  373-7     Citation Subset:  IM    
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MeSH Terms
Carboxy-Lyases / metabolism*
Chloromercuribenzoates / pharmacology
Copper / pharmacology
Dithionitrobenzoic Acid / pharmacology
Ferrous Compounds / pharmacology
Mercury / pharmacology
Metals / pharmacology*
Porphyrins / biosynthesis*
Rhodopseudomonas / metabolism*
Sulfhydryl Compounds / pharmacology*
Uroporphyrinogen Decarboxylase / antagonists & inhibitors,  metabolism*
Zinc / pharmacology
p-Chloromercuribenzoic Acid
Reg. No./Substance:
0/Chloromercuribenzoates; 0/Ferrous Compounds; 0/Metals; 0/Porphyrins; 0/Sulfhydryl Compounds; 59-85-8/p-Chloromercuribenzoic Acid; 69-78-3/Dithionitrobenzoic Acid; 7439-97-6/Mercury; 7440-50-8/Copper; 7440-66-6/Zinc; EC 4.1.1.-/Carboxy-Lyases; EC Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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