| Biosynthesis of poly-beta-hydroxyalkanoate by Brevundimonas vesicularis LMG P-23615 and Sphingopyxis macrogoltabida LMG 17324 using acid-hydrolyzed sawdust as carbon source. | |
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MedLine Citation:
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PMID: 17630126 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poly-beta-hydroxyalkanoate (PHA) is a biodegradable polymer accumulated in intracellular granules by different bacterial species. Its physical and chemical properties are similar to those of petroleum-derived plastics. Material generated by the acid hydrolysis of wood was evaluated for use in the bacterial synthesis of PHA. Acid-hydrolyzed sawdust was prepared and adjusted to pH 7. Mineral salts with carbon:nitrogen (C:N) proportions of 100:1, 100:3.5, 100:10, 100:30, or 100:50 and trace elements were added and these solutions were inoculated with a bacterial strain Brevundimonas vesicularis LMG P-23615 or Sphingopyxis macrogoltabida LMG 17324. The percentage of cells accumulating PHA was evaluated by flow cytometry. The hydrolyzed sawdust composition was analyzed by gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC). The organic material (601.5 mg l(-1)) contained 112.5 mg l(-1) sugars. Over 96% of these sugars were consumed and more than 90% of the bacterial cells accumulated PHA. The 100:3.5 C:N proportion was optimal for growth and PHA synthesis, with yields ranging from 64% to 72% of the dry cell weight. The results suggest that acid-hydrolyzed sawdust can be used by bacteria as a carbon source for growth and PHA production. This forestry by sub-product offers a low-cost alternative for obtaining biodegradable plastics (e.g., PHA) synthesized by bacteria. |
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Authors:
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Johanna A Silva; Lorena M Tobella; José Becerra; Félix Godoy; Miguel A Martínez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 103 ISSN: 1389-1723 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-07-16 Completed Date: 2007-08-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 542-6 Citation Subset: IM |
Affiliation:
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Laboratorio de Microbiología Básica y Biorremediación, Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario S/N, Casilla 160-C, Concepción, Chile. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acids Biodegradation, Environmental Biopolymers / biosynthesis* Carbon / metabolism Caulobacteraceae / metabolism* Hydrolysis Hydroxy Acids / metabolism* Polyesters / metabolism* Sphingomonadaceae / metabolism* Wood |
| Chemical | |
Reg. No./Substance:
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0/Acids; 0/Biopolymers; 0/Hydroxy Acids; 0/Polyesters; 7440-44-0/Carbon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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