Document Detail


Biosynthesis of mono- and sesquiterpenes in carrot roots and leaves (Daucus carota L.): metabolic cross talk of cytosolic mevalonate and plastidial methylerythritol phosphate pathways.
MedLine Citation:
PMID:  15680987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The biosynthesis of the monoterpenes terpinolene and myrcene and the sesquiterpene beta-caryophyllene in roots and leaves of two carrot varieties (Daucus carota L. cultivars Bolero and Kazan) were investigated by in vivo feeding experiments with [5,5-2H2]-mevalonic acid lactone (d2-MVL) and [5,5-2H2]-1-deoxy-D-xylulose (d2-DOX). The volatiles of the tissues were extracted by stir bar sorptive extraction and analyzed using thermal desorption-multidimensional gas chromatography-mass spectrometry. The experiments demonstrate independent de novo-biosynthesis of terpenoids in carrot roots and in carrot leaves. In both plant tissues monoterpenes are biosynthesized exclusively via the 1-deoxy-D-xylulose/2-C-methyl-D-erythritol-4-phosphate (DOXP/MEP) pathway, whereas sesquiterpenes are generated by the classical mevalonic acid pathway as well as by the DOXP/MEP route. A more detailed investigation of carrot root tissues revealed that the biosynthesis of terpenes is mainly localized in the phloem. Nevertheless, in xylem a de novo-biosynthesis of terpenes was detectable as well, even in the absence of oil ducts in this tissue.
Authors:
Daniela Hampel; Armin Mosandl; Matthias Wüst
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytochemistry     Volume:  66     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-31     Completed Date:  2005-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-11     Citation Subset:  IM    
Affiliation:
Institut für Lebensmittelchemie, Johann Wolfgang Goethe-Universität, Marie-Curie-Strasse 9, D-60439 Frankfurt/Main, Germany.
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MeSH Terms
Descriptor/Qualifier:
Cytosol / metabolism
Daucus carota / chemistry,  cytology,  metabolism*
Erythritol / chemistry,  metabolism*
Mass Spectrometry
Mevalonic Acid / chemistry,  metabolism*
Monoterpenes / chemistry,  metabolism*
Plant Leaves / chemistry,  metabolism*
Plant Roots / chemistry,  metabolism*
Plastids / metabolism*
Sesquiterpenes / chemistry,  metabolism*
Stereoisomerism
Chemical
Reg. No./Substance:
0/Monoterpenes; 0/Sesquiterpenes; 149-32-6/Erythritol; 150-97-0/Mevalonic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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