| Biosynthesis of mono- and sesquiterpenes in carrot roots and leaves (Daucus carota L.): metabolic cross talk of cytosolic mevalonate and plastidial methylerythritol phosphate pathways. | |
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MedLine Citation:
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PMID: 15680987 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The biosynthesis of the monoterpenes terpinolene and myrcene and the sesquiterpene beta-caryophyllene in roots and leaves of two carrot varieties (Daucus carota L. cultivars Bolero and Kazan) were investigated by in vivo feeding experiments with [5,5-2H2]-mevalonic acid lactone (d2-MVL) and [5,5-2H2]-1-deoxy-D-xylulose (d2-DOX). The volatiles of the tissues were extracted by stir bar sorptive extraction and analyzed using thermal desorption-multidimensional gas chromatography-mass spectrometry. The experiments demonstrate independent de novo-biosynthesis of terpenoids in carrot roots and in carrot leaves. In both plant tissues monoterpenes are biosynthesized exclusively via the 1-deoxy-D-xylulose/2-C-methyl-D-erythritol-4-phosphate (DOXP/MEP) pathway, whereas sesquiterpenes are generated by the classical mevalonic acid pathway as well as by the DOXP/MEP route. A more detailed investigation of carrot root tissues revealed that the biosynthesis of terpenes is mainly localized in the phloem. Nevertheless, in xylem a de novo-biosynthesis of terpenes was detectable as well, even in the absence of oil ducts in this tissue. |
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Authors:
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Daniela Hampel; Armin Mosandl; Matthias Wüst |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Phytochemistry Volume: 66 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-01-31 Completed Date: 2005-04-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 305-11 Citation Subset: IM |
Affiliation:
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Institut für Lebensmittelchemie, Johann Wolfgang Goethe-Universität, Marie-Curie-Strasse 9, D-60439 Frankfurt/Main, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cytosol
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metabolism Daucus carota / chemistry, cytology, metabolism* Erythritol / chemistry, metabolism* Mass Spectrometry Mevalonic Acid / chemistry, metabolism* Monoterpenes / chemistry, metabolism* Plant Leaves / chemistry, metabolism* Plant Roots / chemistry, metabolism* Plastids / metabolism* Sesquiterpenes / chemistry, metabolism* Stereoisomerism |
| Chemical | |
Reg. No./Substance:
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0/Monoterpenes; 0/Sesquiterpenes; 149-32-6/Erythritol; 150-97-0/Mevalonic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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