| Biosynthesis of long chain hydroxyfatty acids from glucose by engineered Escherichia coli. | |
| | |
MedLine Citation:
|
PMID: 22503196 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
This study devised a pathway in Escherichia coli for direct production of long chain hydroxyfatty acids (HFAs) from glucose. This is first report on the biosynthesis of HFAs from renewable sugar, without the need of exogenous fatty acids. By employing thioesterases BTE and 'TesA to tailor the composition of free fatty acids (FFAs) and using fatty acid hydroxylase P450(BM3) to convert FFAs to HFAs, high-specificity production of C12 and C14 HFAs was achieved. By further knocking out the endogenous fadD gene of E. coli, an engineered strain capable of producing 117.0mg/L HFAs was finally obtained, representing a high HFA production in shake flask. This study indicated an attractive metabolic strategy for the biosynthesis of HFAs directly from renewable carbohydrates resources. |
| | |
Authors:
|
Xiangwei Wang; Lingling Li; Yanning Zheng; Huibin Zou; Yujin Cao; Hui Liu; Wei Liu; Mo Xian |
Related Documents
:
|
9422686 - Engineering broader specificity into an antibiotic-producing polyketide synthase. 22932126 - Folic acid facilitates in vitro maturation of mouse and xenopus laevis oocytes. 23306116 - Effects of pretreatment factors on fermentable sugar production and enzymatic hydrolysi... 23283246 - Triarylsulfonium hexafluorophosphate salts as photoactivated acidic catalysts for ring-... 7476586 - Extracellular nucleic acids as possible messengers in regulatory information transfer f... 9422686 - Engineering broader specificity into an antibiotic-producing polyketide synthase. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-3-4 |
Journal Detail:
|
Title: Bioresource technology Volume: - ISSN: 1873-2976 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
|
Created Date: 2012-4-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Chinese Academy of Sciences, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Graduate University of Chinese Academy of Sciences, Beijing 100049, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nitrite accumulation under constant temperature in anoxic denitrification process: The effects of ca...
Next Document: Curriculum Meeting Points: A transcultural and transformative initiative in nursing education.