Document Detail


Biosynthesis and inactivation of ecdysone during the pupal-adult development of the cabbage butterfly, Pieris brassicae L.
MedLine Citation:
PMID:  7336463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Injection of labelled ecdysone and 2-hydroxyecdysone into Pieris pupae showed that their catabolism proceeds through 26-hydroxylation followed by conversion into acidic steroids assumed to be 26-oic compounds. This biological system is characterized by the lack of conjugation reactions and by rather long-lived hormones. In vivo biosynthesis of ecdysteroids was investigated by 24 hr [3H]cholesterol labelling, followed by HPLC analysis of the resulting [3H]ecdysone and 20-hydroxyecdysone. Active conversion (up to 0.07% in 24 hours) was observed between 48 hr and 120 hr following pupal ecdysis, a result in good agreement with the variations observed in hormone content. Long-term [3H]cholesterol incorporation experiments made it possible to monitor ecdysteroid dynamics during pupal development. Three periods were observed, corresponding to successive accumulation of ecdysone, 20-hydroxyecdysone and an acidic metabolite. Comparison of these results with those of the experiments involving labelled ecdysone injection shows that the catabolism of injected hormones is not the same as that of endogenous hormones.
Authors:
P Beydon; J Claret; P Porcheron; R Lafont
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Steroids     Volume:  38     ISSN:  0039-128X     ISO Abbreviation:  Steroids     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-05-21     Completed Date:  1982-05-21     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  633-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Butterflies / growth & development
Cholesterol / metabolism
Ecdysone / biosynthesis*,  metabolism
Kinetics
Metamorphosis, Biological
Pupa
Chemical
Reg. No./Substance:
3604-87-3/Ecdysone; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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