| Biosynthesis, in vivo, of gonadotropin-releasing hormone in the hypothalamus of normal and ovariectomized female rats. | |
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MedLine Citation:
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PMID: 7035255 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Normal female and ovariectomized rats were infused into the 3rd ventricle with [3H]glycine or [3H]alanine. Some rats were pretreated with cycloheximide. Hypothalamus, anterior pituitary and fragments of cortex were excised, homogenized, extracted and treated with specific antiserum to GnRH, bound to Sepharose. The radioactivity of immuno-absorbed products was counted either immediately of after extraction and thin-layer chromatography by using two different solvent systems. With the two systems, the location of the immuno-absorbed radioactivity always coincided with the spot of synthetic GnRH. Our results show that [3H]glycine was incorporated, as a function of time, into GnRH isolated from rat hypothalami. The amount was incorporated, as a function of time, into GnRH isolated from rat hypothalami. The amount of radioactivity incorporated into hypothalami from diestrous-I rats was similar to that of ovariectomized rats and twice as high as in late proestrous rats. Only minute amounts of radioactivity were incorporated into the immuno-absorbed product. Cycloheximide inhibited incorporation of [3H]glycine into the immuno-absorbed product to the same extent as its incorporation into the total protein from the hypothalamus. Our experimental results support the hypothesis of ribosomal mechanisms being involved in the biosynthesis of GnRH. They also suggest that the rate of accumulation of newly synthesized labeled GnRH is of the same order in the hypothalamus of ovariectomized rats as in diestrous-I rats. |
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Authors:
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K Kochman; B Kerdelhué; A Ostrowska; L Chomicka; E Domanski; M Jutisz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 25 ISSN: 0303-7207 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 1982 Feb |
Date Detail:
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Created Date: 1982-04-12 Completed Date: 1982-04-12 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 193-202 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Animals Castration Chromatography, Thin Layer Cycloheximide / pharmacology Female Hypothalamus / metabolism* Kinetics Pituitary Gland, Anterior / metabolism Pituitary Hormone-Releasing Hormones / biosynthesis*, metabolism Rats |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Pituitary Hormone-Releasing Hormones; 66-81-9/Cycloheximide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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