Document Detail


Biosynthesis of immunoglobulin A (IgA) and immunoglobulin M (IgM). Control of polymerization by J chain.
MedLine Citation:
PMID:  4205353     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell suspensions of mouse plasma-cell tumours secreting IgA (immunoglobulin A) and IgM (immunoglobulin M) were incubated with radioactive leucine for various periods of time. The secreted immunoglobulins were precipitated from the culture medium with specific rabbit antisera to determine the relative distribution of radioactivity among the different molecular species, and to estimate the fraction of total radioactivity in the J chain. For IgM-secreting cells there is a balanced synthesis of 7S subunits and J chains, and the secreted product is uniformly assembled to the pentamer. In cells secreting IgA, however, the results demonstrate that the pool of intracellular J chain is less than the intracellular IgA pool. The concentration of J chain is therefore limiting and is less than the requirement for complete polymerization. The major factor that determines whether an intracellular monomer is secreted as such or is polymerized with the addition of J chain is therefore the amount of intracellular J chain. When this is limiting, as it is in cells secreting IgA, then monomer will be secreted.
Authors:
R M Parkhouse; E Della Corte
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  136     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1973 Nov 
Date Detail:
Created Date:  1974-04-25     Completed Date:  1974-04-25     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  607-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Electrophoresis, Polyacrylamide Gel
Immunoglobulin A / biosynthesis*
Immunoglobulin J-Chains / metabolism*
Immunoglobulin M / biosynthesis*
Leucine / metabolism
Macromolecular Substances
Mice
Neoplasms, Experimental / metabolism
Plasmacytoma / metabolism
Rabbits / immunology
Time Factors
Tritium
Chemical
Reg. No./Substance:
0/Immunoglobulin A; 0/Immunoglobulin J-Chains; 0/Immunoglobulin M; 0/Macromolecular Substances; 10028-17-8/Tritium; 61-90-5/Leucine
Comments/Corrections

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