| Biosynthesis of heparin. O-sulfation of D-glucuronic acid units. | |
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MedLine Citation:
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PMID: 2118524 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Incubation of a microsomal fraction from murine mastocytoma, with UDP-[1-3H]GlcA, UDP-GlcNAc, and adenosine 3'-phosphate 5'-phosphosulfate (PAPS), yielded labeled, N-sulfated polysaccharides, in which most of the incorporated O-sulfate groups were located at C2 of L-iduronic acid and at C6 of D-glucosamine units. Analysis by anion-exchange high pressure liquid chromatography of disaccharides, generated by deaminative cleavage of these polysaccharides, revealed that, in addition, an appreciable portion of the -GlcNSO3-HexA-GlcNSO3- sequences in the intact polymers contained O-sulfated (at C2 or C3) D-glucuronic acid units. Calculations based on such compositional analysis of the N- and O-sulfated biosynthetic product, isolated by chromatography on DEAE-cellulose, showed that glucuronosyl 2/3-O-sulfate accounted for approximately 12% of the total incorporated O-sulfate groups. With [35S]PAPS (at a low total PAPS concentration) as an alternative source of label, the sulfated glucuronic acid residues were again detectable, albeit in much smaller amounts (1.8% of the total O-sulfate groups). Incorporation of label from UDP-[5-3H]GlcA was retained by the O-sulfated glucuronic acid units, thus demonstrating that these components had in fact been formed by sulfation of glucuronic acid residues and not by "back epimerization" of sulfated iduronic acid units. Structural analysis of polysaccharide intermediates at various stages of biosynthetic polymer modification, separated by ion-exchange chromatography, showed O-sulfation of glucuronic and iduronic acid units to appear simultaneously and before the 6-O-sulfation of glucosamine residues. |
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Authors:
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M Kusche; U Lindahl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 265 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1990 Sep |
Date Detail:
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Created Date: 1990-10-11 Completed Date: 1990-10-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 15403-9 Citation Subset: IM |
Affiliation:
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Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrate Sequence Chromatography, Gel Chromatography, High Pressure Liquid Disaccharides / isolation & purification Glucuronates Heparin / biosynthesis* Mast-Cell Sarcoma / metabolism Mice Microsomes / metabolism* Molecular Sequence Data Oligosaccharides / isolation & purification Sulfates / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Disaccharides; 0/Glucuronates; 0/Oligosaccharides; 0/Sulfates; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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