Document Detail


THE BIOSYNTHESIS OF GRAMICIDIN S IN A CELL-FREE SYSTEM.
MedLine Citation:
PMID:  14343149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. A cell-free system prepared from Bacillus brevis cells, harvested in the late phase of growth and consisting of the 11000g supernatant, has been shown to incorporate into gramicidin S the five constituent amino acids added in labelled form. The results are consistent with complete synthesis and not merely a completion of pre-existing intermediate peptides. 2. The incorporation of (14)C-labelled amino acids by the 11000g supernatant into gramicidin S requires an energy source. Omission of phosphoenolpyruvate and pyruvate kinase from the incubation mixture prevents incorporation into gramicidin S. The cell-free system incorporates [(14)C]-leucine, -proline and -phenylalanine over a period of 4hr. With [(14)C]leucine, incorporation into gramicidin S takes place in the range pH6-9 with maximum incorporation at pH7.0. High concentrations of chloramphenicol or puromycin decreased the incorporation into gramicidin S by only about 20%. 3. The 50000g supernatant exhibited no decrease in ability of incorporating [(14)C]valine into gramicidin S as compared with the 11000g supernatant. About 40% of the incorporating ability remained in the 105000g supernatant after 3hr. centrifugation. When recombining the 105000g sediment with the 105000g supernatant, some increase in incorporation over that obtained with the supernatant alone was obtained. The findings tend to support the view that gramicidin S is synthesized in a different manner from that of proteins.
Authors:
T L BERG; L O FROHOLM; S G LALAND
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  96     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1965 Jul 
Date Detail:
Created Date:  1965-12-01     Completed Date:  1996-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  43-52     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate*
Amino Acids / metabolism*
Bacillus*
Chloramphenicol*
Hydrogen-Ion Concentration*
Leucine*
Metabolism*
Ornithine*
Pharmacology*
Phenylalanine*
Proline*
Puromycin*
Pyruvate Kinase*
Pyruvates*
Research*
Tyrothricin*
Valine*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Pyruvates; 1404-88-2/Tyrothricin; 147-85-3/Proline; 53-79-2/Puromycin; 56-65-5/Adenosine Triphosphate; 56-75-7/Chloramphenicol; 61-90-5/Leucine; 63-91-2/Phenylalanine; 7004-03-7/Valine; 7006-33-9/Ornithine; EC 2.7.1.40/Pyruvate Kinase
Comments/Corrections

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