| Biosynthesis of glyoxylate from glycine in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 15851099 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glyoxylate biosynthesis in Saccharomyces cerevisiae is traditionally mainly ascribed to the reaction catalyzed by isocitrate lyase (Icl), which converts isocitrate to glyoxylate and succinate. However, Icl is generally reported to be repressed by glucose and yet glyoxylate is detected at high levels in S. cerevisiae extracts during cultivation on glucose. In bacteria there is an alternative pathway for glyoxylate biosynthesis that involves a direct oxidation of glycine. Therefore, we investigated the glycine metabolism in S. cerevisiae coupling metabolomics data and (13)C-isotope-labeling analysis of two reference strains and a mutant with a deletion in a gene encoding an alanine:glyoxylate aminotransferase. The strains were cultivated on minimal medium containing glucose or galactose, and (13)C-glycine as sole nitrogen source. Glyoxylate presented (13)C-labeling in all cultivation conditions. Furthermore, glyoxylate seemed to be converted to 2-oxovalerate, an unusual metabolite in S. cerevisiae. 2-Oxovalerate can possibly be converted to 2-oxoisovalerate, a key precursor in the biosynthesis of branched-chain amino acids. Hence, we propose a new pathway for glycine catabolism and glyoxylate biosynthesis in S. cerevisiae that seems not to be repressed by glucose and is active under both aerobic and anaerobic conditions. This work demonstrates the great potential of coupling metabolomics data and isotope-labeling analysis for pathway reconstructions. |
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Authors:
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Silas Granato Villas-Bôas; Mats Kesson; Jens Nielsen |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS yeast research Volume: 5 ISSN: 1567-1356 ISO Abbreviation: FEMS Yeast Res. Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-04-26 Completed Date: 2005-12-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101085384 Medline TA: FEMS Yeast Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 703-9 Citation Subset: IM |
Affiliation:
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Center for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alanine Transaminase
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genetics Carbon Isotopes Glycine / metabolism* Glyoxylates / metabolism* Mutation Oxidation-Reduction Saccharomyces cerevisiae / genetics, metabolism* Transaminases / genetics |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Glyoxylates; 298-12-4/glyoxylic acid; 56-40-6/Glycine; EC 2.6.1.-/Transaminases; EC 2.6.1.-/glyoxylate aminotransferase; EC 2.6.1.2/Alanine Transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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