| Biosynthesis of gibberellic acid from milk permeate in repeated batch operation by a mutant Fusarium moniliforme cells immobilized on loofa sponge. | |
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MedLine Citation:
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PMID: 18684618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Gibberellic acid (GA) production from milk permeate was studied by 28 mutants of Fusarium moniliforme, among which mutant gamma-14 was selected as the best producer. Experiments were carried out in shaker flasks and fermentative process was analyzed with free and immobilized cells. Immobilization of mutant gamma-14 cells onto loofa sponge discs was studied with respect to the optimization of the incubation temperature, initial pH, inoculum size (number of discs) and its reusability for GA production. Best yield of GA (2.40 gl(-1)) was recorded by immobilized cells under optimized cultural conditions (4 immobilized discs, 30 degrees C and pH 5). Data obtained during four reusable cycles showed high stability of GA production and reduction in the initiation time of acid production, resulting in higher levels of GA in shorter time duration. Immobilization of mutant gamma-14 cells onto loofa sponge discs, permitted repeated reuse under the specified fermentation conditions for GA production from milk permeate. |
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Authors:
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Samir A Meleigy; Mahmoud A Khalaf |
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Publication Detail:
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Type: Journal Article Date: 2008-08-05 |
Journal Detail:
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Title: Bioresource technology Volume: 100 ISSN: 1873-2976 ISO Abbreviation: Bioresour. Technol. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-09-15 Completed Date: 2008-12-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: England |
Other Details:
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Languages: eng Pagination: 374-9 Citation Subset: IM |
Affiliation:
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Radiation Microbiology Department, National Center for Radiation Research and Technology, Nasr City, Cairo, Egypt. samirelmeligy@hotmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bioreactors / microbiology* Cattle Cells, Immobilized / metabolism Fusarium / classification, genetics*, metabolism* Gibberellins / biosynthesis* Luffa / microbiology* Milk / microbiology* Mutation Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Gibberellins; 77-06-5/gibberellic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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