| Biosynthesis of gastric mucins. In vitro incorporation of radioactive glucose. | |
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MedLine Citation:
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PMID: 130226 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An in vitro model for the study of the mechanism of gastric-glycoprotein (mucin) biosynthesis is described. Gastric mucosa scrapings were incubated in Eagle's minimum medium with U-14C-glucose. Linear incorporation-kinetics were observed during 4 hours. U-14-C-glucose was incorporated into soluble and cell bound glycoproteins. In the acid hydrolysate of soluble and cell bound fractions all the amino acids separated by high voltage electrophoresis and all the carbohydrates separated by thin layer chromatography contained appreciable radioactivity showing the active conversion of the added radioactive glucose to all the amino acid and carbohydrate precursors used by the gastric cells for the biosynthesis of macromolecular glycoprotens. The carbohydrate/protein ratio of the excreted glycoproteins increases steadily during the 4h incubation period suggesting that the chemical composition of the excreted mucins changes during this period. The cell bound glycoprotein fraction retains a constant composition during the 4th incubation period. The high fucose to hexose ratio of the soluble (excreted) glycoproteins suggests that they are probably identical to the gastric mucins isolated from native gastric preparation. This model is therefore considered a valid one for the study of the regulation of the biosynthesis of gastric mucins as well as for the study of drug action. |
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Authors:
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C Decaens; D Waldron-Edward; J P Bader; L Robert |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Connective tissue research Volume: 4 ISSN: 0300-8207 ISO Abbreviation: Connect. Tissue Res. Publication Date: 1975 |
Date Detail:
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Created Date: 1976-06-02 Completed Date: 1976-06-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0365263 Medline TA: Connect Tissue Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 25-30 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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biosynthesis Animals Carbohydrates / analysis, biosynthesis Female Gastric Mucins / analysis, biosynthesis* Gastric Mucosa / metabolism* Glucose / metabolism Glutamates / biosynthesis Hexosamines / analysis Kinetics Male Models, Biological Proteins / analysis Rats |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbohydrates; 0/Gastric Mucins; 0/Glutamates; 0/Hexosamines; 0/Proteins; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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