| Biosynthesis of ferritin in rat hepatoma cells and rat livers. I. Synthesis and assembly of protein subunits of ferritin. | |
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MedLine Citation:
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PMID: 191450 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell fractions were prepared from ACI rat livers and from rat hepatoma cell clone M-5123-C1. Radioimmunoassays of ferritin and of its protein subunits in various cell fractions after biosynthetic labeling with [14C]leucine were done by means of ferritin-specific and subunit-specific rabbit antibody. In both ACI rat livers and M-5123-C1 hepatoma cells free polyribosomes synthesized approximately 81% of the protein subunits of ferritin, and membrane-bound polyribosomes synthesized the rest. In both polyribosomal fractions, [14C]leucine-labeled subunits were detected earlier than [14C]leucine-labeled ferritin and apoferritin (5 min as against 30 min after initiation of a pulse). Time sequence studies of the shifts of biosynthetically labeled subunits and ferritin through different cell compartments provided evidence for vectorial transport of subunits and of ferritin, the direction of transport being from the two polyribosomal systems to the smooth membrane compartment and to the cytosol. |
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Authors:
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S S Lee; G W Richter |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 252 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1977 Mar |
Date Detail:
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Created Date: 1977-05-20 Completed Date: 1977-05-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2046-53 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carcinoma, Hepatocellular / metabolism* Cell Fractionation Endoplasmic Reticulum / metabolism Ferritins / biosynthesis* Kinetics Liver / metabolism* Liver Neoplasms / metabolism* Macromolecular Substances Male Neoplasms, Experimental / metabolism Polyribosomes / metabolism Protein Biosynthesis Rats Rats, Inbred ACI Subcellular Fractions / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Macromolecular Substances; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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