| Biosynthesis of domoic acid by the diatom Pseudo-nitzschia multiseries. | |
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MedLine Citation:
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PMID: 10223629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The biosynthesis of the neurotoxin domoic acid (DA) in the diatom Pseudo-nitzschia multiseries was investigated using 13C- and 14C-labelled precursors. The labelling pattern determined by NMR spectroscopy following incorporation of [1,2-13C2]-acetate showed enrichment of every carbon of DA. The enrichment levels were consistent with a biosynthetic pathway involving two different intermediate precursor units. Addition of labelled acetate either early or late during exponential growth gave similar patterns and levels of incorporation. Analysis of the labelling pattern indicated that DA is biosynthesised by condensation of an isoprenoid intermediate with another intermediate derived from the tricarboxylic acid (TCA) cycle. The absence of deuterium at C2 in DA following incorporation of [2-13C, 2H3]-acetate is consistent with alpha-ketoglutarate or a derivative as the TCA cycle-derived intermediate. The different incorporation efficiencies of acetate into the putative precursor intermediates suggest that either each unit is biosynthesized in a different part of the diatom cell, or that the isoprene chain is not assembled by the usual acetate-mevalonate pathway. The latter proposal is supported by the complete absence of deuterium retention in the isoprenoid-derived portion following incorporation of [2-13C, 2H3]-acetate. |
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Authors:
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U P Ramsey; D J Douglas; J A Walter; J L Wright |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Natural toxins Volume: 6 ISSN: 1056-9014 ISO Abbreviation: Nat. Toxins Publication Date: 1998 |
Date Detail:
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Created Date: 1999-06-16 Completed Date: 1999-06-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9212382 Medline TA: Nat Toxins Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 137-46 Citation Subset: IM |
Affiliation:
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Institute for Marine Biosciences, National Research Council of Canada, Halifax, Nova Scotia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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metabolism Carbon Isotopes Carbon Radioisotopes Diatoms / physiology* Eutrophication Kainic Acid / analogs & derivatives*, chemistry Magnetic Resonance Spectroscopy Marine Toxins / chemistry Neuromuscular Depolarizing Agents / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Carbon Isotopes; 0/Carbon Radioisotopes; 0/Marine Toxins; 0/Neuromuscular Depolarizing Agents; 14277-97-5/domoic acid; 487-79-6/Kainic Acid |
| Comments/Corrections | |
Comment In:
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Nat Toxins. 1998;6(3-4):91-2
[PMID:
10223624
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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