Document Detail

Biosynthesis of docosahexaenoate-containing glycerolipid molecular species in the retina.
MedLine Citation:
PMID:  11478376     Owner:  NLM     Status:  MEDLINE    
Vertebrate retinas are highly enriched in long-chain polyunsaturated fatty acids (PUFA), especially docosahexaenoic acid (22:6n-3, DHA). In the present study, we investigated the role of de novo synthesis in the enrichment of 22:6n-3 in characteristic molecular species of retinal glycerolipids. Following the incubation of fresh dark-adapted retinas with [2-(3)H]-glycerol, individual glycerolipids were isolated and converted into either diacylglycerol acetates (DGAC) or diacylglycerol benzoates (DGBZ), followed by high-performance liquid chromatography (HPLC) and flow-through radioactivity detection. Total lipids from rat retinas incubated with [3H]-glycerol were analyzed. Unlike what was observed with frog retinas, relative larger of amounts of di-22:6 molecular species were synthesized de novo. In both rat and frog retinas, there was synthesis of glycerolipid molecular species containing two PUFA (one of which was 22:6) in larger amounts than predicted by their steady-state mass levels. These results demonstrate that the unique molecular species of retinal glycerolipids are derived only in part through de novo synthesis, but that molecular rearrangement (remodeling) and differential turnover must also play a role in maintaining the high levels of 22:6 found in rod phohtoreceptor outer segments (ROS) membranes.
F Li; H Chen; R E Anderson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of molecular neuroscience : MN     Volume:  16     ISSN:  0895-8696     ISO Abbreviation:  J. Mol. Neurosci.     Publication Date:    2001 Apr-Jun
Date Detail:
Created Date:  2001-07-31     Completed Date:  2002-02-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9002991     Medline TA:  J Mol Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  205-14; discussion 215-21     Citation Subset:  IM    
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA.
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MeSH Terms
Chromatography, High Pressure Liquid
Diglycerides / metabolism
Docosahexaenoic Acids / metabolism*
Glycerol / metabolism
Glycolipids / metabolism*
Molecular Weight
Phosphatidylcholines / metabolism
Phosphatidylethanolamines / metabolism
Rana pipiens
Rats, Sprague-Dawley
Retina / metabolism*
Type C Phospholipases / metabolism
Grant Support
Reg. No./Substance:
0/Diglycerides; 0/Glycolipids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/glycerolglycolipids; 25167-62-8/Docosahexaenoic Acids; 56-81-5/Glycerol; EC 3.1.4.-/Type C Phospholipases

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