Document Detail

Biosynthesis of digalactosyl diglyceride in Vicia faba leaves.
MedLine Citation:
PMID:  1110323     Owner:  NLM     Status:  MEDLINE    
Developing and mature leaf tissue from Vicia faba plants were pulse-fed 14-CO2. The lipids were extracted at intervals after exposure to light, and the galactolipids monogalactosyl diglyceride (MGDG) and digalactosyl diglyceride (DGDG) were separated. After methylation and methanolysis, gas-liquid chromatography was used to separate the two galactose units of DGDG and the galactose of MGDG. The specific activities of the galactoses and the changes over the time period of the experiment were determined. The results support the view that DGDG is formed by galactosylation of MGDG. This does not take place by a rapid two-enzyme system reaction but more slowly in two phases: galactosylation of a pool of newly formed MGDG and a more random galactosylation of MGDG. There is no evidence of a high turnover of galactose in these lipids.
J P Williams; M Khan; S Leung
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  16     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1975 Jan 
Date Detail:
Created Date:  1975-04-14     Completed Date:  1975-04-14     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  61-6     Citation Subset:  IM    
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MeSH Terms
Carbon Dioxide / metabolism
Carbon Radioisotopes
Chlorophyll / metabolism
Chromatography, Gas
Diglycerides / biosynthesis,  metabolism
Fatty Acids / analysis
Galactose / biosynthesis,  metabolism
Glycolipids / biosynthesis*,  metabolism
Plants / enzymology,  growth & development,  metabolism*
Time Factors
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Diglycerides; 0/Fatty Acids; 0/Glycolipids; 124-38-9/Carbon Dioxide; 1406-65-1/Chlorophyll; 26566-61-0/Galactose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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