Document Detail

Biosynthesis of chloramphenicol in Streptomyces species 3022a: the nature of the arylamine synthetase system.
MedLine Citation:
PMID:  583399     Owner:  NLM     Status:  MEDLINE    
The arylamine synthetase which catalyses the conversion of chorismic acid to p-aminophenylalanine in Streptomyces species 3022a was separated from aminotransferase by DEAE-cellulose chromatography. Recovered activity was further fragmented into three separate components by passage through a Sephadex G-100 column. Only one component produced p-aminophenylalanine but in combination the three stimulated each other activity. Although the products of the other two components were unstable, an infrared spectrum of one of them was obtained and confirmed the presence of an aromatic amine, but other functional groups could not be ascertained. This product was not recognized as a substrate by the arylamine synthetase complex and it was suggested that it may be a degradation product of an intermediate of p-aminophenylalanine biosynthesis or an unknown intermediate of later biosynthetic steps of the chloramphenicol pathway. It is further suggested that acrylamine compounds produced by this organism are the result of interaction of a core protein with other macromolecules and small molecular weight effect or molecules.
M M Francis; D W Westlake
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  25     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-05-14     Completed Date:  1980-05-14     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1408-15     Citation Subset:  IM    
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MeSH Terms
Anthranilate Synthase / metabolism
Chloramphenicol / biosynthesis*
Chromatography, Gel
Glutaminase / metabolism
Molecular Weight
Multienzyme Complexes / metabolism*
Streptomyces / enzymology*
Reg. No./Substance:
0/Multienzyme Complexes; 55467-45-3/arylamine synthetase; 56-75-7/Chloramphenicol; EC; EC Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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