Document Detail

Biosynthesis of caffeic acid in Escherichia coli using its endogenous hydroxylase complex.
Jump to Full Text
MedLine Citation:
PMID:  22475509     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ABSTRACT: BACKGROUND: Caffeic acid (3,4-dihydroxycinnamic acid) is a natural phenolic compound derived from the plant phenylpropanoid pathway. Caffeic acid and its phenethyl ester (CAPE) have attracted increasing attention for their various pharmaceutical properties and health-promoting effects. Nowadays, large-scale production of drugs or drug precursors via microbial approaches provides a promising alternative to chemical synthesis and extraction from plant sources. RESULTS: We first identified that an Escherichia coli native hydroxylase complex previously characterized as the 4-hydroxyphenylacetate 3-hydroxylase (4HPA3H) was able to convert p-coumaric acid to caffeic acid efficiently. This critical enzymatic step catalyzed in plants by a membrane-associated cytochrome P450 enzyme, p-coumarate 3-hydroxylase (C3H), is difficult to be functionally expressed in prokaryotic systems. Moreover, the performances of two tyrosine ammonia lyases (TALs) from Rhodobacter species were compared after overexpression in E. coli. The results indicated that the TAL from R. capsulatus (Rc) possesses higher activity towards both tyrosine and L-dopa. Based on these findings, we further designed a dual pathway leading from tyrosine to caffeic acid consisting of the enzymes 4HPA3H and RcTAL. This heterologous pathway extended E. coli native tyrosine biosynthesis machinery and was able to produce caffeic acid (12.1 mg/L) in minimal salt medium. Further improvement in production was accomplished by boosting tyrosine biosynthesis in E. coli, which involved the alleviation of tyrosine-induced feedback inhibition and carbon flux redirection. Finally, the titer of caffeic acid reached 50.2 mg/L in shake flasks after 48-hour cultivation. CONCLUSION: We have successfully established a novel pathway and constructed an E. coli strain for the production of caffeic acid. This work forms a basis for further improvement in production, as well as opens the possibility of microbial synthesis of more complex plant secondary metabolites derived from caffeic acid. In addition, we have identified that TAL is the rate-limiting enzyme in this pathway. Thus, exploration for more active TALs via bio-prospecting and protein engineering approaches is necessary for further improvement of caffeic acid production.
Authors:
Yuheng Lin; Yajun Yan
Related Documents :
238469 - Extracellular lipids of cladosporium (amorphotheca) resinae grown on glucose or on n-al...
22319559 - Tissue-specific whole transcriptome sequencing in castor, directed at understanding tri...
18293369 - Self-assembled polyhydroxy fatty acids vesicles: a mechanism for plant cutin synthesis.
8250229 - Analysis of lipids in crude extracts by 13c nuclear magnetic resonance.
9248619 - Mode of action of glycolic acid on human stratum corneum: ultrastructural and functiona...
11539709 - Lipid content and fatty acid composition of green algae scenedesmus obliquus grown in a...
9288919 - Structure of the o-specific polysaccharide of proteus vulgaris o25 containing 3-o-[(r)-...
21166269 - Reduced salicylic acid binding following noise: possible evidence for prestin disruption.
6954419 - A comparative study of polylactic acid, gelfoam, and surgicel in healing extraction sites.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-4
Journal Detail:
Title:  Microbial cell factories     Volume:  11     ISSN:  1475-2859     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139812     Medline TA:  Microb Cell Fact     Country:  -    
Other Details:
Languages:  ENG     Pagination:  42     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Microb Cell Fact
Journal ID (iso-abbrev): Microb. Cell Fact
ISSN: 1475-2859
Publisher: BioMed Central
Article Information
Download PDF
Copyright ©2012 Lin and Yan; licensee BioMed Central Ltd.
open-access:
Received Day: 18 Month: 1 Year: 2012
Accepted Day: 4 Month: 4 Year: 2012
collection publication date: Year: 2012
Electronic publication date: Day: 4 Month: 4 Year: 2012
Volume: 11First Page: 42 Last Page: 42
ID: 3379962
Publisher Id: 1475-2859-11-42
PubMed Id: 22475509
DOI: 10.1186/1475-2859-11-42

Biosynthesis of caffeic acid in Escherichia coli using its endogenous hydroxylase complex
Yuheng Lin1 Email: linyh@uga.edu
Yajun Yan2 Email: yajunyan@uga.edu
1Department of Biological and Agricultural Engineering, the University of Georgia, Athens, GA 30602, USA
2Biochemical Engineering Program, Faculty of Engineering, the University of Georgia, Athens, GA 30602, USA

Background

Caffeic acid (3,4-dihydroxycinnamic acid) is a natural phenolic compound initially found in plants. Previous studies on its biological activities suggested that caffeic acid possesses anti-oxidant [1,2], anti-virus [3], anti-cancer [4] and anti-inflammatory properties [5]. Moreover, its derivative, caffeic acid phenethyl ester (CAPE), has drawn great attention because of its demonstrated therapeutic effects including its potential as an anti-diabetic and liver-protective agent as well as an anti-tumor drug for human breast cancer treatment [6,7].

Caffeic acid is one of the pivotal intermediates of plant phenylpropanoid pathway starting from the deamination of phenylalanine which generates cinnamic acid. Followed by a two-step sequential hydroxylation at the 4- and 3- position of the benzyl ring, cinnamic acid is converted into caffeic acid via p-coumaric acid [8,9]. The involved enzymes, cinnamate 4-hydroxylase (C4H) and p-coumarate 3-hydroxylase (C3H) are plant-specific cytochrome P450 dependent monooxygenases. Due to their instability and membrane-bound property, the purification and characterization of these enzymes are quite challenging, particularly for C3H [10]. It was also suggested that the hydroxylation at the 3-position could also occur after p-coumaric acid is esterified, which does not generate caffeic acid as the intermediate [8,11]. Recently, genes and enzymes involved in caffeic acid biosynthesis were also reported in the actinomycete Saccharothrix espanaensis. A tyrosine ammonia lyase (TAL) encoded by sam8 and a microbial C3H encoded by sam5 are responsible for the conversion of tyrosine to p-coumaric acid and then to caffeic acid, respectively [12].

Currently, caffeic acid is commonly produced by extraction from plant sources, such as coffee beans. Chemical or enzymatic hydrolysis of caffeoylquinic acid derivatives is also employed to produce caffeic acid [13,14]. Like many other secondary metabolites, caffeic acid derivatives are usually accumulated at low levels in plants and hence the isolation of these compounds is to some extent difficult and expensive. Microbial conversion provides an alternative approach to caffeic acid production. Sachan et al. reported the co-production of caffeic acid and p-hydroxybenzoic acid in Streptomyces caeruleus by feeding p-coumaric acid [15]. Over decades, advances in metabolic engineering and synthetic biology enable the production of various plant-specific secondary metabolites in recombinant microorganisms [16-19]. Most recently, the conversion of tyrosine to caffeic acid (the titer was not reported) and ferulic acid (7.1 mg/L) in E. coli was achieved by the co-expression of the enzymes encoded by the sam5 and sam8 from S. espanaensis and an O-methyltransferase from Arabidopsis thaliana [20] . However, the above-mentioned studies relied on feeding the direct precursors such as tyrosine and p-coumaric acid, which would increase the production cost and can not be preferred for large-scale production. Alternatively, the development of processes that can enable the biosynthesis of these high-value metabolites from simple carbon sources is much more desirable. By utilizing tyrosine-overproducing strains as hosts, the production of several natural compounds such as L-dopa, flavonoids, and benzylisoquinoline alkaloids from simple carbon sources has already been achieved [21-23].

In this study, we characterized the E. coli native 4-hydroxyphenlacetate 3-hydroxylase (4HPA3H) that was capable of hydroxylating p-coumaric acid and tyrosine in addition to its native substrate 4-hydroxyphenylacetic acid. Moreover, we found the TAL from Rhodobacter capsulatus was able to accept both tyrosine and L-dopa as substrates. Based on these findings, we further designed a novel dual pathway leading from tyrosine to caffeic acid mediated by the enzymes 4HPA3H and TAL. As shown in Figure 1B, native tyrosine biosynthesis can be extended by the introduction of the 4HPA3H and TAL, yielding L-dopa and p-coumaric acid, respectively. Then TAL further converts L-dopa to caffeic acid; while 4HPA3H converts p-coumaric acid into caffeic acid as well. Furthermore, by grafting this dual pathway into E. coli, we successfully achieved de novo biosynthesis of caffeic acid. This work not only opens the route to the production of caffeic acid from simple carbon sources, but also paves the way to the microbial synthesis of many other phenylpropanoids derived from caffeic acid.


Results and discussion

Plants and bacteria are very different in cell structure, physiology and genetics. One of the difficulties in reconstructing plant pathways in microbial systems is the availability of functional enzymes that are compatible with the specific microorganism. For the biosynthesis of caffeic acid in plants, two cytochrome P450-dependent monooxygenases are involved, which are C4H and C3H [10]. Due to the requirement for anchorage on endoplasmic reticulum, functional expression of these plant P450-dependent enzymes were always problematic in bacterial systems [10,24]. Fortunately, TALs identified from various sources can catalyze the direct formation of p-coumaric acid from tyrosine bypassing the enzymatic step catalyzed by C4H [25], and thus, the need for a C4H was not obligatory. Nevertheless, the need for C3H still remains. Although an alternative microbial C3H was identified from S. espanaensis, its activity seems to be low, which limits its applications [20].

p-Coumaric Acid Hydroxylation by 4HPA3H

One of the most challenging steps in reconstructing plant phenylpropanoid pathway in E. coli is the 3-hydroxylation of p-coumaric acid, because all C3Hs identified in plants are cytochrome P450-dependent monooxygenases and are hard to be functionally expressed in bacterial systems [10]. Therefore, the exploration of alternative enzymes compatible with E. coli is necessary. By examining E. coli native enzymes and pathways related to metabolism of aromatic compounds, we reasoned that the 4HPA3H complex encoded by the operon hpaBC involved in the 4-hydroxyphenylacetate (4-HPA) degradation may play the role of C3H [26,27]. This enzyme complex can accept a broad range of substrates and has been applied to produce L-dopa and hydroxytyrosol from tyrosine and 4-tyrosol, respectively [23,26-28]. Because p-coumaric acid is similar to 4-HPA, tyrosine and 4-tyrosol in molecular structure (Figure 2), we reasoned that the catalytic pocket of 4HPA3H should be able to accommodate p-coumaric acid as well.

To test this hypothesis, we first cloned hpaBC into a high-copy expression vector pZE12-luc. After over-expressing this enzyme complex in wild type E. coli BW25113, crude extract was prepared for in vitro enzyme assay. Our results indicate that 4HPA3H complex is capable of converting p-coumaric acid to caffeic acid in the presence of flavin adenine dinucleotide (FAD) and nicotinamide adenine dinucleotide hydride (NADH) (Table 1). Its specific activity toward p-coumaric acid (5.37 × 10-3 U/mg protein) is much higher than its activity toward tyrosine (2.44 × 10-3 U/mg protein).

Furthermore, we carried out whole-cell conversion studies which reflect the in vivo enzymatic activity. BW25113 harboring pZE-EcHpaBC was able to completely convert 100 mg/L p-coumaric acid to caffeic acid within 3 hours after the induction of isopropyl β-D-1- thiogalactopyranoside (IPTG), indicating the in vivo activity toward p-coumaric acid is high. Meanwhile, no caffeic acid was detected in the culture of the control strain (BW25113 harboring pZE12-luc) even after 20 hours. This phenomenon suggested that although hpaBC exists in the genome of E. coli, it is not natively expressed. Thus, over-expression of hpaBC is necessary to obtain adequate 4HPA3H activity. The result of in vivo enzyme assay showed that the highest conversion rates (within the first hour) from tyrosine to L-dopa and from p-coumaric acid to caffeic acid are 112.98 and 240.80 μmol· h-1·gDCW-1, respectively (Figure 3). For both products, we did not observe obvious intracellular accumulation. Both in vitro and in vivo assay results indicate that p-coumaric acid is preferred by 4HPA3H. To our knowledge, this is the first report of the 4HPA3H activity toward p-coumaric acid.

Comparison of RcTAL and RsTAL

Previous studies reported that TALs from R. capsulatus (Rc) and R. sphaeroides (Rs) catalyze the deamination of tyrosine [25]. In addition, RsTAL can also take L-dopa as a substrate [29]. But the activity of RcTAL toward L-dopa has not been investigated. To evaluate the performance of the two TALs in E. coli, we performed in vitro enzyme assays using crude extracts. The genes encoding the two TALs were cloned and expressed in E. coli using the plasmids pZE-RcTAL and pZE-RsTAL. Interestingly, both TALs slightly prefer L-dopa over their native substrate tyrosine. For RcTAL, the specific activities toward tyrosine and L-dopa were 0.93 × 10-3 and 1.54 × 10-3 U/mg protein, respectively. For RsTAL, the specific activities are 0.80 × 10-3 and 1.14 × 10-3 U/mg protein, respectively. The results indicated that RcTAL is slightly more active than RsTAL toward both substrates (Table 2). As a control, the crude extract of the wild-type E. coli carrying the blank vector did not exhibit any activity.

Production of caffeic Acid in E. coli

Based on the activities of 4HPA3H and RcTAL, we proposed a novel dual pathway for caffeic acid biosynthesis from tyrosine (Figure 1B). Because E. coli natively biosynthesizes tyrosine, it is expected that the introduction of RcTAL and 4HPA3H can result in the biosynthesis of caffeic acid by utilizing E. coli endogenous tyrosine (Figure 1). To achieve this goal, the genes encoding RcTAL and 4HPA3H were amplified and consecutively cloned into a high-copy-number plasmid pZE12-luc under the control of a strong IPTG-inducible promoter PLlacO1, generating the plasmid pZE-TH. A ribosome binding site (RBS) was placed upstream of each gene. Strain YL-2 was developed by introducing pZE-TH into wild type E. coli strain BW25113 to test this pathway. The production of caffeic acid was carried out in shake flasks using modified M9 minimal salt medium as described in "Methods and Materials". High performance liquid chromatography (HPLC) analysis of the fermentation samples showed that the retention time (10.1 min) and UV profile of the product were identical to those of the caffeic acid standard, confirming that caffeic acid was produced (Figure 4). The strain YL-2 was able to produce 11.1 ± 1.1 mg/L caffeic acid after 24 hours, without obvious accumulation of intermediates including tyrosine, p-coumaric acid and L-dopa. 48-hour cultivation did not lead to a great increase in caffeic acid production (12.1 ± 0.3 mg/L) (Table 3). However, L-dopa was accumulated at a concentration of 7.4 ± 0.2 mg/L.

Construction of tyrosine overproducers

The addition of 100 mg/L tyrosine into the cultures of YL-2 resulted in a two-fold increase in caffeic acid production (Table 3), suggesting that tyrosine is a limiting precursor for caffeic acid biosynthesis. In wild-type E. coli, tyrosine biosynthesis is strictly controlled by several regulatory mechanisms. Two feedback inhibition-sensitive enzymes chorismate mutase-prephenate dehydrogenase (CM-PDH, encoded by tyrA) and 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS, encoded by aroG) were identified as the major regulatory components in the tyrosine pathway [30]. The feedback inhibition resistant (fbr) variants tyrAfbr and aroGfbr have already been developed and applied in tyrosine production [30,31]. In addition, the availability of erythrose-4-phosphate (E4P) and phosphoenolpyruvate (PEP) is extremely critical to tyrosine biosynthesis (Figure 1A). Over-expression of PEP synthase (PPS, encoded by ppsA) and transketolase (TKT, encoded by tktA) was able to increase the availability of PEP and E4P, and redirect the carbon flux into the tyrosine pathway [31]. In this work, tyrAfbr, ppsA, tktA and aroGfbr were consecutively cloned into a medium-copy-number plasmid pCS27 under the control of PLlacO1 promoter as well, generating the plasmid pCS-TPTA. By introducing pCS-TPTA into wild type E. coli BW25113, we obtained a recombinant strain YL-3. Compared with wild type strain which produced little tyrosine, YL-3 was able to produce 296.6 ± 1.0 mg/L tyrosine in 48 hours, which indicated that over-expression of the four enzymes was effective. Furthermore, the strain YL-1 (ΔtyrR) was also employed as the host to alleviate the tyrR-mediated regulation [23]. The introduction of pCS-TPTA into YL-1 (yielding strain YL-4) resulted in the accumulation of higher amount of tyrosine (426.7 ± 4.9 mg/L in 48 h, Table 3). It should be noted that YL-4 exhibited only slight improvement in tyrosine production compared to YL-3 in the first 24 hours. However, its advantage was demonstrated in the following 24 hours. These results are consistent with what were reported previously [30,31].

Improvement of caffeic acid production by tyrosine overproducing strains

Although YL-4 was able to produce higher amount of tyrosine, this tyrR-deleted strain seemed to be in conflict with pZE-derived plasmids for unknown reasons and did not express the enzymes 4HPA3H and RcTAL as well as expected. Only a trace amount of caffeic acid (< 0.2 mg/L) and p-coumaric acid (< 1 mg/L) but a large amount of tyrosine (> 400 mg/L) were detected in the YL-6 (YL-1 harboring pZE-TH and pCS-TPTA) cultures. Thus, we employed wild type E. coli BW25113 as the parent strain. By transforming it with both pZE-TH and pCS-TPTA, we generated the strain YL-5. The titer of caffeic acid in the shake flask fermentation using YL-5 reached 50.2 ± 10.1 mg/L after 48 h fermentation which is a 5-fold increase compared to YL-2. Moreover, we analyzed the intermediates accumulated in the culture. The presence of 25.1 ± 2.5 mg/L tyrosine indicated that tyrosine availability is no longer the limiting factor for caffeic acid production in the strain YL-5. The accumulation of a large amount of L-dopa (75.3 ± 13.6 mg/L) and a small amount of coumaric acid (< 0.2 mg/L) suggested that RcTAL became into the rate-limiting step in this artificial pathway, especially after 24 h (Table 3).


Conclusions

We have successfully established a novel pathway and constructed an E. coli strain for the de novo production of caffeic acid via metabolic engineering approaches. We first identifed that 4HPA3H can function as a C3H which exhibited decent activity toward p-coumaric acid and tyrosine, thus gains great potential for metabolic engineering and biocatalysis applications. In addition, we compared the TALs from R. capsulatus (Rc) and R. sphaeroids (Rs) that are able to catalyze the deamination of both tyrosine and L-dopa. RcTAL exhibited higher activities toward both substrates. Then a dual pathway leading from tyrosine to caffeic acid was proposed and introduced into E. coli. The artificial pathway extended the native tyrosine pathway of E. coli and produced 12.1 mg/L of caffeic acid from simple carbon sources. Further improvement of production was accomplished via alleviating feedback inhibition and redirecting carbon flux into tyrosine biosynthesis. Finally, the titer of caffeic acid reached 50.2 mg/L in shake flasks after 48-hour cultivation.

The established pathway obviated the use of two cytochrome P450-dependent monooxygenases (C4H and C3H) and achieved the de novo biosynthesis of caffeic acid, which opened the possibility of microbial synthesis of more complex plant secondary metabolites derived from caffeic acid. However, for the production system to be more economically viable, productivity has to be further improved. We have identified that RcTAL is the rate-limiting enzyme in the pathway once the tyrosine availability issue was solved. To meet the process metrics and avoid the accumulation of the intermediates (tyrosine and L-dopa), we will explore more TALs for higher catalytic activity via bioprospecting and protein engineering approaches. In addition to the tyrosine overproducers we generated in this study, the strains employed to produce tyrosine in amino acid industry are also ready to be used as hosts for caffeic acid production. With proper process optimization, industrially relevant production should be expectable.


Materials and methods
Chemicals and enzymes

The following commercially available chemicals and enzymes were used in this study: L-dopa (ACROS Organics); tyrosine (Sigma-Aldrich), caffeic acid (TCI), p-coumaric acid (MP Biochemicals), IPTG (Zymo Research Co.), restriction enzymes (NEB), Hot Start KOD Plus DNA polymerase (EMD Chemicals Inc.), Rapid DNA ligase Kit (Roche). All the enzymes were used according to the instructions provided by the manufacturers.

Strains, plasmids, media, and growth conditions

E. coli XL1-Blue (Stratagene) was used for gene cloning and plasmid propagation. Wild type E. coli strain BW25113 (E. coli Genetic Resource Center) and its derivatives were employed for either enzyme assays or shake flask experiments. Plasmids pZE12-luc and pCS27 were used for gene over-expression in E. coli [32,33]. The characteristics of all the strains and plasmids used in this study are described in Table 4. E. coli cells for gene cloning, plasmid propagation, and inoculum preparation were grown in Luria-Bertani (LB) medium at 37°C. The fermentation medium was modified M9 minimal salt medium containing (per liter): glycerol (10 g), glucose (2.5 g), NH4Cl (1 g), Na2HPO4 (6 g), KH2PO4 (3 g), NaCl (0.5 g), MgSO4·7H2O (2 mmol), CaCl2·2H2O (0.1 mmol), vitamin B1 (2.0 mg), H3BO3 (1.25 mg), NaMoO4·2H2O (0.15 mg), CoCl2·6H2O (0.7 mg), CuSO4·5H2O (0.25 mg), MnCl2·4H2O (1.6 mg), and ZnSO4·7H2O (0.3 mg). For the strains carrying plasmids, 100 μg/ml of ampicillin, 50 μg/ml of kanamycin and/or 30 μg/ml of chloramphenicol were added if necessary. For all shake flask experiments, 200 μl overnight LB culture was inoculated into 10 ml fermentation medium and grown at 37°C with shaking. After OD600 reached 0.4-0.5, IPTG was added into the cultures to a final concentration of 0.2 mM. Then the cultures were transferred to 30°C in a gyratory shaker at 250 rpm. Samples were collected after 24 and 48 hours, and then analyzed by HPLC.

Molecular biology techniques

General molecular biology techniques and DNA manipulations were carried out according to the standard protocols [34]. Deletion of kanamycin resistant gene from E. coli JW1316-1 was conducted using the method described by Kirill A. Datsenko and Barry L. Wanner [35]. Host cells were transformed with the plasmids by electroporation (EPPENDORF Electroporator 2510, 1.8 kV when using 0.1 cm cuvettes).

Construction of plasmids

To construct pZE-RcTAL and pZE-RsTAL, the genes encoding RcTAL and RsTAL were amplified by high-fidelity polymerase chain reaction (PCR) from the genomic DNAs of Rhodobacter capsulatus and Rhodobacter sphaeroides using the primers listed in Additional file 1: Table S1 [25]. Amplified fragments and pZE12-luc were digested with KpnI and SphI, and then ligated with Rapid DNA ligase. To construct pZE-EcHpaBC, the gene cluster hpaBC was amplified from E. coli MG1655 genome directly. The amplified hpaBC fragment was inserted into pZE12-luc vector between KpnI and SphI as well. The pZE-TH was constructed by cloning the gene cluster hpaBC into the pZE-RcTAL using restriction enzymes SphI and XbaI. A ribosome binding site is located upstream of each gene to facilitate protein expression. The genes tyrA, aroG, ppsA, and tktA were all amplified from E. coli MG1655 genomic DNA. Point mutations were introduced to tyrA (Met-53-Ile and Ala-354-Val) and aroG (Asp-146-Asn) by splicing and overlapping extension PCR (SOE-PCR), generating tyrAfbr and aroGfbr [36,37]. The genes tyrAfbr and ppsA were first cloned into pCS27 simultaneously via three-piece ligation using restriction enzymes KpnI, NdeI, and SalI, generating the plasmid pCS-TP. Similarly, tktA and aroGfbr were then simultaneously inserted into pCS-TP using restriction enzymes XhoI, SphI, and HindIII resulting in pCS-TPTA.

4HPA3H In vitro assay

The E. coli strain BW25113 carrying the plasmid pZE-EcHpaBC was pre-inoculated into LB liquid medium containing 100 μg/ml of ampicillin and grown at 37°C overnight with shaking at 250 rpm. In the following day, 1 ml of preinoculum was added to 50 ml of fresh LB medium also containing 100 μg/ml of ampicillin. The culture was left to grow at 37°C till OD600 reached 0.6 and then induced with 0.5 mM IPTG. Protein expression was conducted at 30°C for another 3 h. The cells were harvested and resuspended in 2 ml of buffer A (20 mM KH2PO4, pH = 7.0), and then lysed by French Press. The soluble fraction was collected by ultra-centrifugation and used as crude enzyme extract for the enzyme assay. Total protein concentration was estimated using the BCA kit (Pierce Chemicals). The total protein concentration of the crude extract is around 6172 μg/ml. The enzyme activity was assayed according to the protocol described by Tai. et al. with a few modifications [26]. The 1 ml reaction system contained 2 mM NADH, 2 mM FAD, 2 mM substrate (tyrosine or p-coumaric acid) and 100 μl of crude enzyme extract in buffer A. The reaction was incubated at 30°C for 1.5 min and terminated by adding 50 μl HCl (20%) to the 1 ml reaction system. The amount of products (L-dopa and caffeic acid, respectively) were measured and quantified by HPLC.

Whole-cell Bioconversion by 4HPA3H

The E. coli strain BW25113 carrying the plasmid pZE-EcHpaBC was pre-inoculated into LB liquid medium containing 100 μg/ml of ampicillin and grown at 37°C overnight with shaking at 250 rpm. Then 0.1 ml of preinoculum was added to 10 ml of fresh LB medium also containing 100 μg/ml of ampicillin. The culture was grown at 37°C till OD600 reached 0.6 and then induced with 0.5 mM IPTG for 3 hours. After that, 100 uL of p-coumaric acid (10 g/L) was added to reach a final concentration of 100 mg/L. Samples were collected at 3 h and analyzed by HPLC

4HPA3H In vivo assay

The pre-inoculum of E. coli strain BW25113 carrying pZE-EcHpaBC from an overnight culture was added in to 10 ml of LB medium (1:100 V/V) and grown at 37°C. IPTG was added to the cultures to a final concentration of 0.5 mM until OD600 reached 0.6. The cultures were left at 30°C for around another 3 hours with shaking for protein expression till OD600 reached 3.0 (approximately equivalent to 1 g/L cell). Then the cells were collected, washed, resuspended in 10 ml of NaCl (0.9%) solution. 1 mM substrate (tyrosine or p-coumaric acid) was added to the cell resuspensions at 30°C. Samples were collected after 1 and 2 hours, and then analyzed by HPLC.

Enzyme assay of RcTAL and RsTAL

The crude enzyme extracts of RcTAL and RsTAL were prepared as described before [25]. But the cells were resuspended in buffer B (50 mM, Tris-HCl, pH = 8.5). The 1 ml reaction system contained 2 mM substrate (tyrosine or L-dopa) and 100 μl crude extract in buffer B. The reaction was incubated at 30°C for 1.5 min and the amount of products (p-coumaric acid and caffeic acid, respectively) was measured by HPLC.

HPLC analysis of products

Tyrosine, L-dopa, p-coumaric acid, and caffeic acid generated in enzyme assays and fermentations were quantitatively analyzed by HPLC (Dionex Ultimate 3000) with a reverse-phase ZORBAX SB-C18 column and an Ultimate 3000 Photodiode Array Detector. The compounds were separated by elution with a methanol-water gradient (water containing 0.2% trifluoroacetic acid). The following gradient was used at a flow rate of 1 ml/min: 10 to 50% methanol for 15 min, 50 to 10% methanol for 1 min, and 10% methanol for an additional 4 min. Quantification for the four above-mentioned compounds was based on the peak areas of absorbance at 274, 280, 308 and 323 nm, respectively. The data shown in this study were generated from duplicate or triplicate independent experiments.


Competing interests

The University of Georgia has filed a United State provisional patent on this technology.


Authors' contributions

YY and YL conceived the study. YL conducted the experiments under the direction of YY. YL did literature review and drafting of the manuscript. YY made revisions. Both authors read and approved the final manuscript.


Supplementary Material Additional file 1

Table S1 Primers used in this study.


Click here for additional data file (1475-2859-11-42-S1.DOC)


Acknowledgements

This work was supported by the start-up funds from the Faculty of Engineering, University of Georgia, Athens and a research grant provided by the University of Georgia Research Foundation, Inc. We would like to thank Rachit Jain for proofreading the manuscript.


References
Mori H,Iwahashi H,Antioxidant activity of caffeic acid through a novel mechanism under uva irradiationJ Clin Biochem NutrYear: 200945495510.3164/jcbn.08-25819590707
Gulcin I,Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid)ToxicologyYear: 200621721322010.1016/j.tox.2005.09.01116243424
Ikeda K,Tsujimoto K,Uozaki M,Nishide M,Suzuki Y,Koyama AH,Yamasaki H,Inhibition of multiplication of herpes simplex virus by caffeic acidInt J Mol MedYear: 20112859559821725588
Rajendra-Prasad N,Karthikeyan A,Karthikeyan S,Reddy BV,Inhibitory effect of caffeic acid on cancer cell proliferation by oxidative mechanism in human HT-1080 fibrosarcoma cell lineMol Cell BiochemYear: 2011349111910.1007/s11010-010-0655-721116690
Chao PC,Hsu CC,Yin MC,Anti-inflammatory and anti-coagulatory activities of caffeic acid and ellagic acid in cardiac tissue of diabetic miceNutr Metab (Lond)Year: 200963310.1186/1743-7075-6-3319678956
Wu J,Omene C,Karkoszka J,Bosland M,Eckard J,Klein CB,Frenkel K,Caffeic acid phenethyl ester (CAPE), derived from a honeybee product propolis, exhibits a diversity of anti-tumor effects in pre-clinical models of human breast cancerCancer LettYear: 2011308435310.1016/j.canlet.2011.04.01221570765
Celik S,Erdogan S,Tuzcu M,Caffeic acid phenethyl ester (CAPE) exhibits significant potential as an antidiabetic and liver-protective agent in streptozotocin-induced diabetic ratsPharmacol ResYear: 20096027027610.1016/j.phrs.2009.03.01719717012
Bourgaud F,Hehn A,Larbat R,Doerper S,Gontier E,Kellner S,Matern U,Biosynthesis of coumarins in plants: a major pathway still to be unravelled for cytochrome P450 enzymesPhytochem RevYear: 2006529330810.1007/s11101-006-9040-2
Kojima M,Takeuchi W,Detection and characterization of p-coumaric acid hydroxylase in mung bean, Vigna mungo, seedlingsJ BiochemYear: 19891052652702498300
Kim YH,Kwon T,Yang HJ,Kim W,Youn H,Lee JY,Youn B,Gene engineering, purification, crystallization and preliminary X-ray diffraction of cytochrome P450 p-coumarate-3-hydroxylase (C3H), the Arabidopsis membrane proteinProtein Expr PurifYear: 20117914915510.1016/j.pep.2011.04.01321549841
Kneusel RE,Matern U,Nicolay K,Formation of trans-caffeoyl-CoA from trans-4-coumaroyl-CoA by Zn2 + -dependent enzymes in cultured plant cells and its activation by an elicitor-induced pH shiftArch Biochem BiophysYear: 198926945546210.1016/0003-9861(89)90129-X2919878
Berner M,Krug D,Bihlmaier C,Vente A,Muller R,Bechthold A,Genes and enzymes involved in caffeic acid biosynthesis in the actinomycete Saccharothrix espanaensisJ BacteriolYear: 20061882666267310.1128/JB.188.7.2666-2673.200616547054
Wang J,Lu DQ,Zhao H,Ling XQ,Jiang B,Ouyang PK,Application of response surface methodology optimization for the production of caffeic acid from tobacco wasteAf J BiotechnolYear: 2009814161424
Yoshimoto M,Kurata-Azuma R,Fujii M,Hou DX,Ikeda K,Yoshidome T,Osako M,Enzymatic production of caffeic acid by koji from plant resources containing caffeoylquinic acid derivativesBiosci Biotechnol BiochemYear: 2005691777178110.1271/bbb.69.177716195601
Sachan A,Ghosh S,Sen SK,Mitra A,Co-production of caffeic acid and p-hydroxybenzoic acid from p-coumaric acid by Streptomyces caeruleus MTCC 6638Appl Microbiol BiotechnolYear: 20067172072710.1007/s00253-005-0197-116292647
Yan Y,Chemler J,Huang L,Martens S,Koffas MA,Metabolic engineering of anthocyanin biosynthesis in Escherichia coliAppl Environ MicrobiolYear: 2005713617362310.1128/AEM.71.7.3617-3623.200516000769
Yan Y,Kohli A,Koffas MA,Biosynthesis of natural flavanones in Saccharomyces cerevisiaeAppl Environ MicrobiolYear: 2005715610561310.1128/AEM.71.9.5610-5613.200516151160
Yan Y,Huang L,Koffas MA,Biosynthesis of 5-deoxyflavanones in microorganismsBiotechnol JYear: 200721250126210.1002/biot.20070011917806100
Yan Y,Li Z,Koffas MA,High-yield anthocyanin biosynthesis in engineered Escherichia coliBiotechnol BioengYear: 200810012614010.1002/bit.2172118023053
Choi O,Wu CZ,Kang SY,Ahn JS,Uhm TB,Hong YS,Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coliJ Ind Microbiol BiotechnolYear: 2011381657166510.1007/s10295-011-0954-321424580
Santos CN,Koffas M,Stephanopoulos G,Optimization of a heterologous pathway for the production of flavonoids from glucoseMetab EngYear: 20111339240010.1016/j.ymben.2011.02.00221320631
Nakagawa A,Minami H,Kim JS,Koyanagi T,Katayama T,Sato F,Kumagai H,A bacterial platform for fermentative production of plant alkaloidsNat CommunYear: 2011232621610729
Munoz AJ,Hernandez-Chavez G,de Anda R,Martinez A,Bolivar F,Gosset G,Metabolic engineering of Escherichia coli for improving L -3,4-dihydroxyphenylalanine (L -DOPA) synthesis from glucoseJ Ind Microbiol BiotechnolYear: 2011381845185210.1007/s10295-011-0973-021512819
Leonard E,Runguphan W,O'Connor S,Prather KJ,Opportunities in metabolic engineering to facilitate scalable alkaloid productionNat Chem BiolYear: 2009529230010.1038/nchembio.16019377455
Xue Z,McCluskey M,Cantera K,Sariaslani FS,Huang L,Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroidesJ Ind Microbiol BiotechnolYear: 20073459960410.1007/s10295-007-0229-117602252
Louie TM,Xie XS,Xun L,Coordinated production and utilization of FADH2 by NAD(P)H-flavin oxidoreductase and 4-hydroxyphenylacetate 3-monooxygenaseBiochemistryYear: 2003427509751710.1021/bi034092r12809507
Prieto MA,Perez-Aranda A,Garcia JL,Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate rangeJ BacteriolYear: 1993175216221678458860
Liebgott PP,Amouric A,Comte A,Tholozan JL,Lorquin J,Hydroxytyrosol from tyrosol using hydroxyphenylacetic acid-induced bacterial cultures and evidence of the role of 4-HPA 3-hydroxylaseRes MicrobiolYear: 200916075776610.1016/j.resmic.2009.09.01519837158
Noel JP,Louie GV,Bowman ME,Moore BS,Moffitt MC,Substrate switched ammonia lyases and mutasesUnited States Patent ApplicationYear: 2009 No. 2009/0011400 A1.
Chavez-Bejar MI,Lara AR,Lopez H,Hernandez-Chavez G,Martinez A,Ramirez OT,Bolivar F,Gosset G,Metabolic engineering of Escherichia coli for L-tyrosine production by expression of genes coding for the chorismate mutase domain of the native chorismate mutase-prephenate dehydratase and a cyclohexadienyl dehydrogenase from Zymomonas mobilisAppl Environ MicrobiolYear: 2008743284329010.1128/AEM.02456-0718344329
Lutke-Eversloh T,Stephanopoulos G,L-tyrosine production by deregulated strains of Escherichia coliAppl Microbiol BiotechnolYear: 20077510311010.1007/s00253-006-0792-917221195
Shen CR,Liao JC,Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathwaysMetab EngYear: 20081031232010.1016/j.ymben.2008.08.00118775501
Lutz R,Bujard H,Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elementsNucleic Acids ResYear: 1997251203121010.1093/nar/25.6.12039092630
Sambrook J,Fritsch EF,Maniatis T,Molecular cloning: a laboratory manualYear: 19892NY: Cold Spring Harbor Laboratory
Datsenko KA,Wanner BL,One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsProc Natl Acad Sci USAYear: 2000976640664510.1073/pnas.12016329710829079
Heckman KL,Pease LR,Gene splicing and mutagenesis by PCR-driven overlap extensionNat ProtocYear: 2007292493210.1038/nprot.2007.13217446874
Lutke-Eversloh T,Stephanopoulos G,Feedback inhibition of chorismate mutase/prephenate dehydrogenase (TyrA) of Escherichia coli: generation and characterization of tyrosine-insensitive mutantsAppl Environ MicrobiolYear: 2005717224722810.1128/AEM.71.11.7224-7228.200516269762

Figures

[Figure ID: F1]
Figure 1 

Proposed caffeic acid biosynthetic pathway. (A) Native tyrosine biosynthetic pathway in E. coli. (B) The artificial dual pathway mediated by 4HPA3H and TAL for caffeic acid biosynthesis from tyrosine. PPS: phosphoenolpyruvate synthase; TKT: transketolase; CM-PDH: chorismate mutase-prephenate dehydrogenase; DAHPS: 3-deoxy-D-arabino- heptulosonate-7-phosphate synthase; 4HPA3H: 4-hydroxyphenylacetate 3-hydroxylase; TAL: tyrosine ammonia lyase; E4P: D-erythrose-4-phosphate; PEP: phosphoenolpyruvate; HPP: 4-hydroxyphenylpyruvate.



[Figure ID: F2]
Figure 2 

Molecular structures of p-coumaric acid and three known substrates of 4HPA3H. The circles indicate the hydroxylation positions. The boxes indicate the difference of molecular structures.



[Figure ID: F3]
Figure 3 

In vivo enzyme activity of 4HPA3H complex toward tyrosine and p-coumaric acid. The grey and black bars refer to the amount of caffeic acid and L-dopa, respectively.



[Figure ID: F4]
Figure 4 

HPLC analysis of caffeic acid produced by engineered E. coli (A) Standard, 50 mg/L caffeic acid. (B) A sample taken from the fermentation culture of YL-2 after 24 hours. Peak 1 corresponded to caffeic acid. The retention time was 10.1 min. UV absorbance profiles are shown beside the peaks.



Tables
[TableWrap ID: T1] Table 1 

In vitro activity of 4HPA3H complex


Enzyme Activity toward Substrate Ratio (A : B)
A (tyrosine)
(10-3 U/mg protein)
B (p-coumaric acid)
(10-3 U/mg protein)

4HPA3H 2.44 ± 0.11 5.37 ± 0.31 0.45

One U (unit) is defined as the amount (1 μmole) of product formed per minute.


[TableWrap ID: T2] Table 2 

Comparison of in vitro activity of RcTAL and RsTAL


Enzyme Activity toward Substrate* Ratio (A : C)
A (tyrosine)
(10-3 U/mg protein)
C (L-dopa)
(10-3 U/mg protein)

RcTAL 0.93 ± 0.03 1.54 ± 0.05 0.60
RsTAL 0.80 ± 0.13 1.14 ± 0.03 0.70

* The crude extract of the wild-type E. coli did not show any TAL activity


[TableWrap ID: T3] Table 3 

Production of caffeic acid and tyrosine by engineered E.coli strains


Strain 24 hours 48 hours

Product
(mg/L)
Intermediates
(mg/L)
Product
(mg/L)
Intermediates
(mg/L)


caffeic acid tyrosine p-coumaric acid L-dopa caffeic
acid
tyrosine p-coumaric acid L-dopa
YL-2 11.1 ± 1.1 < 0.2 < 0.2 < 0.2 12.1 ± 0.3 < 0.2 < 0.2 7.4 ± 0.2
YL-2* 20.2 ± 1.8 < 0.2 < 0.2 2.1 ± 0.3 21.5 ± 4.0 < 0.2 < 0.2 14.2 ± 1.7
YL-3 - 188.9 ± 5.7 - - - 296.6 ± 1.0 - -
YL-4 - 218.6 ± 8.2 - - - 426.7 ± 4.9 - -
YL-5 30.5 ± 1.9 17.1 ± 1.8 2.9 ± 0.3 15.4 ± 1.7 50.2 ± 10.1 25.1 ± 2.5 < 0.2 75.3 ± 13.6

-: No production

*: addition of 100 mg/L tyrosine in the cultures


[TableWrap ID: T4] Table 4 

Strains and plasmids used in this study


Plasmid or Strain Relevant characteristics Source
Plasmids
pZE12-luc ColE1 ori; AmpR; PLlacO1; luc Lutz et al., 1997
pCS27 p15A ori; KanR; PLlacO1; MCS1 Shen et al., 2008
pZE-RcTAL From pZE12, PLlacO1; tal(Rc) This study
pZE-RsTAL From pZE12, PLlacO1; tal(Rs) This study
pZE-EcHpaBC From pZE12, PLlacO1; hpaB(Ec)-hpaC(Ec) This study
pZE-TH From pZE12, PLlacO1; tal(Rc)-hpaB(Ec)-hpaC(Ec) This study
pCS-TPTA From pCS27, PLlacO1; tyrAfbr-ppsA-tktA-aroGfbr This study
Strains
XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F' proAB lacIqZ ΔM15 Tn10 (TetR)] Stratagene
BW25113 F-, Δ(araD-araB), ΔlacZ (::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB), hsdR Yale CGSC
JW1316-1 BW25113, ΔtyrR::kan Yale CGSC
YL-1 BW25113, ΔtyrR::FRT (as JW1316-1, but kanR gene deleted) This study
YL-2 BW25113 harboring pZE-TH This study
YL-3 BW25113 harboring pCS-TPTA This study
YL-4 YL-1 harboring pCS-TPTA This study
YL-5 BW25113 harboring pZE-TH and pCS-TPTA This study
YL-6 YL-1 harboring pZE-TH and pCS-TPTA This study


Article Categories:
  • Research


Previous Document:  Increasing student involvement and learning through using debate as an assessment.
Next Document:  Cloning and characterization of three chemosensory proteins from Spodoptera exigua and effects of ge...