Document Detail

Biosynthesis of the branched-chain amino acids in the cyanobacterium Synechocystis PCC6803: existence of compensatory pathways.
MedLine Citation:
PMID:  12070685     Owner:  NLM     Status:  MEDLINE    
Complementation of an E. coli mutant auxotrophic for the branched-chain amino acids (BCAA)--valine, leucine, and isoleucine--by the ilvG gene ( slr2088) of the cyanobacterium Synechocystis PCC6803 indicates that this gene encodes an active alpha-acetohydroxy acid synthase. Differences of response of the recombinants to the addition of the essential amino acids suggested a lower specificity for the initial reaction of the valine/leucine chain than for the isoleucine one. Inactivation of ilvG in Synechocystis led to a leaky phenotype, suggesting a capacity to compensate the auxotrophies by other processes. This observation is discussed in view of the general difficulty of obtaining auxotrophs in cyanobacteria.
Odile Maestri-El Kouhen; Françoise Joset
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current microbiology     Volume:  45     ISSN:  0343-8651     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-06-18     Completed Date:  2003-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  94-8     Citation Subset:  IM    
Laboratoire de Chimie Bactérienne-CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
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MeSH Terms
Amino Acids, Branched-Chain / biosynthesis*
Cyanobacteria / genetics,  metabolism*
Escherichia coli / genetics
Genetic Complementation Test
Isoleucine / metabolism
Leucine / metabolism
Valine / metabolism
Reg. No./Substance:
0/Amino Acids, Branched-Chain; 61-90-5/Leucine; 7004-03-7/Valine; 73-32-5/Isoleucine

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