| Biosynthesis and bioactivity of trichosanthin in cultured crown gall tissues of Trichosanthes kirilowii Maximowicz. | |
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MedLine Citation:
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PMID: 16758196 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Trichosanthin (TCS) from Trichosanthes kirilowii Maximowicz (T. kirilowii) can be used to treat choriocarcinoma. In this work, we established a novel system to produce TCS in crown gall tissues of T. kirilowii infected by Agrobacterium tumefaciens C58 (A. tumefaciens). In the crown gall tissues, a nopaline synthase (NOS) gene of A. tumefaciens was identified by polymerase chain reaction (PCR), and nopaline accumulation was confirmed by a high-voltage filter paper electrophoresis. Furthermore, we optimized conditions to culture the crown gall tissues able to grow fast and produce TCS in an auxin-free medium, and found that a fungal elicitor of Armillaria mellea was capable of stimulation of TCS secretion into the medium. Moreover, we identified that the TCS purified from the crown gall tissues could induce gastric cancer cell death. These data underscore the usefulness of our system as an inexpensive and virtually unlimited source of TCS. |
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Authors:
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Hetian Lei; Jianjun Qi; Jingyuan Song; Dejun Yang; Yanzhi Wang; Yinling Zhang; Junshan Yang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-07 |
Journal Detail:
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Title: Plant cell reports Volume: 25 ISSN: 0721-7714 ISO Abbreviation: Plant Cell Rep. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-10-20 Completed Date: 2007-02-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9880970 Medline TA: Plant Cell Rep Country: Germany |
Other Details:
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Languages: eng Pagination: 1205-12 Citation Subset: IM |
Affiliation:
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Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, Hai-Dian District, Beijing, 100094, PR China. Leihetian18@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Oxidoreductases
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genetics Antineoplastic Agents, Phytogenic / isolation & purification, therapeutic use* Basidiomycota / genetics Bioreactors Cell Line, Tumor Cell Survival / drug effects Humans Plant Tumors / genetics*, microbiology Rhizobium radiobacter / genetics Trichosanthes / growth & development, metabolism* Trichosanthin / biosynthesis*, isolation & purification, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 60318-52-7/Trichosanthin; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.5.1.-/nopaline synthase |
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