Document Detail

Biosynthesis and bioactivity of trichosanthin in cultured crown gall tissues of Trichosanthes kirilowii Maximowicz.
MedLine Citation:
PMID:  16758196     Owner:  NLM     Status:  MEDLINE    
Trichosanthin (TCS) from Trichosanthes kirilowii Maximowicz (T. kirilowii) can be used to treat choriocarcinoma. In this work, we established a novel system to produce TCS in crown gall tissues of T. kirilowii infected by Agrobacterium tumefaciens C58 (A. tumefaciens). In the crown gall tissues, a nopaline synthase (NOS) gene of A. tumefaciens was identified by polymerase chain reaction (PCR), and nopaline accumulation was confirmed by a high-voltage filter paper electrophoresis. Furthermore, we optimized conditions to culture the crown gall tissues able to grow fast and produce TCS in an auxin-free medium, and found that a fungal elicitor of Armillaria mellea was capable of stimulation of TCS secretion into the medium. Moreover, we identified that the TCS purified from the crown gall tissues could induce gastric cancer cell death. These data underscore the usefulness of our system as an inexpensive and virtually unlimited source of TCS.
Hetian Lei; Jianjun Qi; Jingyuan Song; Dejun Yang; Yanzhi Wang; Yinling Zhang; Junshan Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-07
Journal Detail:
Title:  Plant cell reports     Volume:  25     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-20     Completed Date:  2007-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1205-12     Citation Subset:  IM    
Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Xi-Bei-Wang, Hai-Dian District, Beijing, 100094, PR China.
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MeSH Terms
Amino Acid Oxidoreductases / genetics
Antineoplastic Agents, Phytogenic / isolation & purification,  therapeutic use*
Basidiomycota / genetics
Cell Line, Tumor
Cell Survival / drug effects
Plant Tumors / genetics*,  microbiology
Rhizobium radiobacter / genetics
Trichosanthes / growth & development,  metabolism*
Trichosanthin / biosynthesis*,  isolation & purification,  pharmacology
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 60318-52-7/Trichosanthin; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.5.1.-/nopaline synthase

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