Document Detail

Biosynthesis of beta-glucuronides of retinol and of retinoic acid in vivo and in vitro.
MedLine Citation:
PMID:  4295866     Owner:  NLM     Status:  MEDLINE    
After the intraportal injection of retinol-6,7-(14)C to rats, the O-ether derivative of retinol, retinyl -glucosiduronate, appears in the bile. Both retinoyl -glucuronide and retinyl -glucosiduronate are also synthesized in vitro when washed rat liver microsomes are incubated with uridine diphosphoglucuronic acid (UDPGA) and either retinoic acid or retinol, respectively. The synthesis of retinoyl -glucuronide was also demonstrated in microsomes of the kidney and in particulate fractions of the intestinal mucosa. The glucuronides were characterized by their UV absorption spectra, by their quenching of UV light or fluorescence under it, by their thin-layer chromatographic behavior in two solvent systems, and by the identification of products released during their hydrolysis by -glucuronidase. With retinoic acid as the substrate, the UDP glucuronyl transferase of rat liver microsomes had a pH optimum of 7.0, a temperature optimum of 38 degrees C, and a marked dependence on the concentrations of both retinoic acid and UDPGA, but was unaffected by a number of possible inhibitors, protective agents, and competitive substrates. The conversion of retinal to retinoic acid and the synthesis of retinoyl -glucuronide from retinoic acid could not be detected in whole homogenates, cell fractions, or outer segments of the bovine retina.
K Lippel; J A Olson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  9     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1968 Mar 
Date Detail:
Created Date:  1968-05-03     Completed Date:  1968-05-03     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  168-75     Citation Subset:  IM    
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MeSH Terms
Bile / analysis
Bile Ducts / physiology
Carbon Isotopes
Chromatography, Thin Layer
Glucuronates / biosynthesis*
Intestines / metabolism*
Kidney / metabolism*
Liver / metabolism*
Microsomes / metabolism
Mitochondria / metabolism
Silicon Dioxide
Vitamin A / metabolism*
Reg. No./Substance:
0/Carbon Isotopes; 0/Glucuronates; 11103-57-4/Vitamin A; 7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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