Document Detail

Biosynthesis of beta-glucans by cell-free extracts from Saccharomyces cerevisiae.
MedLine Citation:
PMID:  22355     Owner:  NLM     Status:  MEDLINE    
Cell-free extracts from Saccharomyces cerevisiae catalyzed the incorporation of glucosyl residues from UDP-[U-14C]glucose into beta-1,3-glucans which contained a significant proportion of beta-1,6-glycosidic linkages. When GDP-[U-14C]glucose was used as substrate only trace amounts of glucose were incorporated. Activity of beta-glucan synthetase was distributed among membrane and cell wall fractions, specific activity being higher in this latter. Beta-glucan synthesized by membrane and cell wall fractions contained 0.6% and 2.5% of beta-1,6-glycosidic linkages respectively. A marked decrease in the activity of beta-glucan synthetase occurred as the cells aged. Significant activity of glycogen synthetase was detected only in cells which had reached the stationary phase of growth.
E López-Romero; J Ruiz-Herrera
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  500     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1977 Dec 
Date Detail:
Created Date:  1978-02-18     Completed Date:  1978-02-18     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  372-84     Citation Subset:  IM    
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MeSH Terms
Cell Cycle
Cell-Free System
Glucosyltransferases / metabolism
Glycogen Synthase / metabolism
Hydrogen-Ion Concentration
Molecular Conformation
Polysaccharides / biosynthesis*
Saccharomyces cerevisiae / metabolism*
Subcellular Fractions / enzymology
Reg. No./Substance:
0/Polysaccharides; EC 2.4.1.-/Glucosyltransferases; EC Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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