| Biosynthesis of benzoylformic acid from benzoyl cyanide by a newly isolated Rhodococcus sp. CCZU10-1 in toluene-water biphasic system. | |
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MedLine Citation:
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PMID: 22033370 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Benzoylformic acid was synthesized from the hydrolysis of benzoyl cyanide by a newly isolated Rhodococcus sp. CCZU10-1. In this study, an aqueous-toluene biphasic system was developed for highly efficient production of benzoylformic acid from the hydrolysis of benzoyl cyanide. In the aqueous-toluene biphasic system, the phase volume ratio, buffer pH and reaction temperature were optimized. Using fed-batch method, a total of 932mM benzoylformic acid accumulated in the reaction mixture after the 10th feed. Moreover, enzymatic hydrolysis of benzoyl cyanide using calcium alginate entrapped resting cells was carried out in the aqueous-toluene biphasic system, and efficient biocatalyst recycling was achieved as a result of cell immobilization in calcium alginate, with a product-to-biocatalyst ratio of 14.26g benzoylformic acid g(-1) dry cell weight (DCW) cell after 20 cycles of repeated use. |
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Authors:
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Yu-Cai He; Qiong Zhou; Cui-Luan Ma; Zhi-Qiang Cai; Li-Qun Wang; Xi-Yue Zhao; Qun Chem; Da-Zhou Gao; Ming Zheng; Xu-Dong Wang; Quan Sun |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-25 |
Journal Detail:
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Title: Bioresource technology Volume: - ISSN: 1873-2976 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-10-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Laboratory of Biochemical Engineering, College of Pharmaceutical and Life Sciences, Changzhou University, Changzhou 213164, China; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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