| Biosynthesis of bacillomycin D by Bacillus subtilis. Evidence for amino acid-activating enzymes by the use of affinity chromatography. | |
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MedLine Citation:
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PMID: 1644198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacillomycin D is an antifungal lipopeptide produced by B. subtilis. The formation of the peptidyl bonds of bacillomycin D occurs non-ribosomally, as demonstrated by the use of chloramphenicol, an inhibitor of protein biosynthesis. Amino acid-activating enzymes were found in B. subtilis cell lysates purified by affinity chromatography on a gel containing L-Pro, an amino acid of bacillomycin D. Presence of ATP during this purification increases the binding of enzymatic proteins and their activity. An enzyme, with an apparent molecular weight of 230 kDa, catalyzed ATP-PPi exchange reactions, which were mediated by specific amino acids, corresponding to a partial sequence of bacillomycin D. |
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Authors:
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F Besson; G Michel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 308 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1992-09-09 Completed Date: 1992-09-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 18-21 Citation Subset: IM |
Affiliation:
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Laboratoire de Biochimie Microbienne (CNRS UMR 24), Université Claude-Bernard Lyon I, Villeurbanne, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Amino Acid Sequence Amino Acyl-tRNA Synthetases / isolation & purification, metabolism* Anti-Bacterial Agents* / biosynthesis* Bacillus subtilis / metabolism* Chromatography, Affinity Chromatography, Gel Molecular Sequence Data Molecular Weight Peptides* Peptides, Cyclic / biosynthesis Transfer RNA Aminoacylation |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Peptides; 0/Peptides, Cyclic; 56-65-5/Adenosine Triphosphate; 76012-17-4/bacillomycin D; EC 6.1.1.-/Amino Acyl-tRNA Synthetases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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