Document Detail

Biosynthesis of acyl groups on molecular species of biliary phosphatidylcholines during metabolism of [2,2,2-2H3]ethanol.
MedLine Citation:
PMID:  1182137     Owner:  NLM     Status:  MEDLINE    
Incorporation of deuterium into different positions of individual molecular species of biliary phosphatidylcholines was determined in bile fistula rats given [2,2,2-2H3]ethanol under conditions ensuring maximal rate of oxidation for 24 h. The deuterium-labelling of the glycerol moiety of the major molecular species was about 6-8 atom% at the end of ethanol administration. The deuterium excess at each of the different positions of the glycerol moiety of 1-palmitoyl-2-linoleoyl phosphatidylcholine was less than 3 atom%. From the isotopic composition of the palmitoyl residues of the phosphatidylcholines, it was calculated that [2,2,2-2H3]ethanol supplied about 35-40% of the acetyl-CoA forming the terminal methyl group and about 25-30% of the other C2 units of the palmitic acid chain. This difference in deuterium incorporation was interpreted as being due to an isotope effect, probably in the rate-limiting carboxylation step of acetyl-CoA. Most or perhaps all of the acetyl groups derived from ethanol were introduced into the terminal methyl group without loss of deuterium. This indicates that citrate is not an important carrier of acetyl-CoA in the biosynthesis of fatty acids from ethanol.
T Curstedt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  398     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1975 Aug 
Date Detail:
Created Date:  1976-01-10     Completed Date:  1976-01-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  265-74     Citation Subset:  IM    
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MeSH Terms
Bile / metabolism*
Ethanol / pharmacology*
Fatty Acids / biosynthesis*
Glycerol / biosynthesis
Linoleic Acids / biosynthesis
Oleic Acids / biosynthesis
Palmitic Acids / biosynthesis
Phosphatidylcholines / biosynthesis*
Phospholipids / metabolism
Time Factors
Reg. No./Substance:
0/Fatty Acids; 0/Linoleic Acids; 0/Oleic Acids; 0/Palmitic Acids; 0/Phosphatidylcholines; 0/Phospholipids; 56-81-5/Glycerol; 64-17-5/Ethanol; 7782-39-0/Deuterium

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